PDF(Pubmed)
Abstract：
Streptomyces species are attractive sources of secondary metabolites that serve as major sources of antibiotics and other drugs. In this study, genome mining was used to determine the biosynthetic potential of Streptomyces sp. 21So2-11 isolated from Antarctic soil. 16S rRNA gene sequencing revealed that this strain is most closely related to Streptomyces drozdowiczii NBRC 101007T, with a similarity of 98.02%. Genome comparisons based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) showed that strain 21So2-11 represents a novel species of the genus Streptomyces. In addition to a large number of genes related to environmental adaptation and ecological function, a total of 28 putative biosynthetic gene clusters (BGCs) responsible for the biosynthesis of known and/or novel secondary metabolites, including terpenes, lantipeptides, polyketides, nonribosomal peptides, RiPPs and siderophores, were detected in the genome of strain 21So2-11. In addition, a total of 1456 BGCs were predicted to contribute to the biosynthesis of more than 300 secondary metabolites based on the genomes of 47 Streptomyces strains originating from polar regions. The results indicate the potential of Streptomyces sp. 21So2-11 for bioactive secondary metabolite production and are helpful for understanding bacterial adaptability and ecological function in cold terrestrial environments.
摘要：
链霉菌属是次生代谢产物的有吸引力的来源，是抗生素和其他药物的主要来源。在这项研究中,基因组挖掘用于确定链霉菌的生物合成潜力。21So2-11分离自南极土壤。16SrRNA基因测序显示，该菌株与drozdowiczii链霉菌NBRC101007T最密切相关，相似度为98.02%。基于平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）的基因组比较表明，菌株21So2-11代表链霉菌属的新物种。除了大量与环境适应和生态功能相关的基因,共有28个推定的生物合成基因簇（BGC）负责已知和/或新的次级代谢产物的生物合成，包括萜烯,抗肽,聚酮化合物,非核糖体肽，RiPP和铁载体，在菌株21So2-11的基因组中检测到。此外,根据来自极地地区的47个链霉菌菌株的基因组，预计总共有1456个BGC有助于300多种次生代谢产物的生物合成。结果表明链霉菌的潜力。21So2-11用于生物活性次级代谢产物的生产，有助于了解细菌在寒冷的陆地环境中的适应性和生态功能。
