Abstract：
Guanine nucleotide-binding proteins of the ADP ribosylation factor (Arf) family and their activating proteins (Arf-GAPs) are essential for diverse biological processes. Here, two homologous Arf-GAPs, Age1 (AoAge1) and Age2 (AoAge2), were identified in the widespread nematode-trapping fungus Arthrobotrys oligospora. Our results demonstrated that AoAge1, especially AoAge2, played crucial roles in mycelial growth, sporulation, trap production, stress response, mitochondrial activity, DNA damage, endocytosis, reactive oxygen species production, and autophagy. Notably, transcriptome data revealed that approximately 62.7% of the genes were directly or indirectly regulated by AoAge2, and dysregulated genes in Aoage2 deletion were enriched in metabolism, ribosome biogenesis, secondary metabolite biosynthesis, and autophagy. Furthermore, Aoage2 inactivation caused a substantial reduction in several compounds compared to the wild-type strain. Based on these results, a regulatory network for AoAge1 and AoAge2 was proposed and verified using a yeast two-hybrid assay. Based on our findings, AoAge1 and AoAge2 are essential for vegetative growth and mycelial development. Specifically, AoAge2 is required for sporulation and trapping morphogenesis. Our results demonstrated the critical functions of AoAge1 and AoAge2 in mycelial growth, diverse cellular processes, and pathogenicity, offering deep insights into the functions and regulatory mechanisms of Arf-GAPs in nematode-trapping fungi.
摘要：
ADP核糖基化因子（Arf）家族的鸟嘌呤核苷酸结合蛋白及其激活蛋白（Arf-GAP）对于多种生物过程至关重要。这里,两个同源Arf-GAP，Age1(AoAge1)和Age2(AoAge2),在广泛的线虫诱捕真菌Arthrobotrys寡孢子中被发现。我们的结果表明，AoAge1，尤其是AoAge2，在菌丝生长中起着至关重要的作用。孢子形成,圈闭生产,应激反应，线粒体活性，DNA损伤,内吞作用,活性氧的产生，和自噬。值得注意的是,转录组数据显示，大约62.7%的基因被AoAge2直接或间接调控，Aoage2缺失中失调的基因在代谢中富集，核糖体生物发生，次级代谢产物生物合成，和自噬。此外，与野生型菌株相比，Aoage2失活导致几种化合物的大幅减少。基于这些结果,提出了AoAge1和AoAge2的监管网络，并使用酵母双杂交测定法进行了验证。根据我们的发现,AoAge1和AoAge2对营养生长和菌丝体发育至关重要。具体来说,AoAge2是孢子形成和捕获形态发生所必需的。我们的结果表明AoAge1和AoAge2在菌丝体生长中的关键功能，不同的细胞过程，和致病性，深入了解Arf-GAP在线虫诱捕真菌中的功能和调控机制。
