Abstract：
This study addresses the increasing concern regarding cyanotoxin contamination of water bodies, highlighting the diversity of these toxins and their potential health implications. Cyanobacteria, which are prevalent in aquatic environments, produce toxic metabolites, raising concerns regarding human exposure and associated health risks, including a potential increase in cancer risk. Although existing research has primarily focused on well-known cyanotoxins, recent technological advancements have revealed numerous unknown cyanotoxins, necessitating a comprehensive assessment of multiple toxin categories. To enhance the cyanotoxin databases, we optimized the CyanoMetDB cyanobacterial secondary metabolites database by incorporating secondary fragmentation patterns using the Mass Frontier fragmentation data prediction software. Water samples from diverse locations in Shanghai were analyzed using high-resolution mass spectrometry. Subsequently, the toxicity of cyanobacterial metabolites in the water samples was examined through acute toxicity assays using the crustacean Thamnocephalus platyurus. After 24 h of exposure, the semi-lethal concentrations (LC50) of the water samples ranged from 0.31 mg L-1 to 1.78 mg L-1 (MC-LR equivalent concentration). Our findings revealed a critical correlation between the overall concentration of cyanobacterial metabolites and toxicity. The robust framework and insights of this study underscore the need for an inclusive approach to water quality management, emphasizing continuous efforts to refine detection methods and comprehend the broader ecological impact of cyanobacterial blooms on aquatic ecosystems.
摘要：
这项研究解决了对水体中氰基毒素污染的日益关注，强调这些毒素的多样性及其潜在的健康影响。蓝细菌,在水生环境中普遍存在，产生有毒的代谢物，引起人们对人类暴露和相关健康风险的关注，包括癌症风险的潜在增加。尽管现有的研究主要集中在众所周知的氰毒素上，最近的技术进步揭示了许多未知的氰基毒素，需要对多种毒素类别进行全面评估。为了加强氰毒素数据库，我们使用MassFrontier碎片数据预测软件,通过纳入二次碎片模式,优化了CyanoMetDB蓝藻次生代谢产物数据库.使用高分辨率质谱分析了上海不同地区的水样。随后，使用甲壳动物Thamnocalusplatyurus通过急性毒性测定法检查了水样中蓝细菌代谢物的毒性。暴露24小时后，水样的半致死浓度（LC50）范围为0.31mgL-1至1.78mgL-1（MC-LR当量浓度）。我们的发现揭示了蓝细菌代谢物的总浓度与毒性之间的关键相关性。这项研究的强大框架和见解强调了对水质管理采取包容性方法的必要性，强调不断努力完善检测方法，了解蓝藻水华对水生生态系统的更广泛生态影响。
