PDF(Pubmed)
Abstract：
Fusarium pseudograminearum causes destructive crown disease in wheat. The velvet protein family is a crucial regulator in development, virulence, and secondary metabolism of fungi. We conducted a functional analysis of FpVelB using a gene replacement strategy. The deletion of FpVelB decreased radial growth and enhanced conidial production compared to that of wild type. Furthermore, FpVelB modulates the fungal responses to abiotic stress through diverse mechanisms. Significantly, virulence decreased after the deletion of FpVelB in both the stem base and head of wheat. Genome-wide gene expression profiling revealed that the regulation of genes by FpVelB is associated with several processes related to the aforementioned phenotype, including \"immune\", \"membrane\", and \"antioxidant activity\", particularly with regard to secondary metabolites. Most importantly, we demonstrated that FpVelB regulates pathogen virulence by influencing deoxynivalenol production and modulating the expression of the PKS11 gene. In conclusion, FpVelB is crucial for plant growth, asexual development, and abiotic stress response and is essential for full virulence via secondary metabolism in F. pseudograminearum.
摘要：
假镰刀菌引起小麦的破坏性冠病。天鹅绒蛋白家族是发育中的关键调节剂，毒力,和真菌的次生代谢。我们使用基因置换策略对FpVelB进行了功能分析。与野生型相比，FpVelB的缺失降低了径向生长并增强了分生孢子的产生。此外，FpVelB通过多种机制调节真菌对非生物胁迫的应答。重要的是,小麦茎基部和头部的FpVelB缺失后，毒力降低。全基因组基因表达谱显示，FpVelB对基因的调控与上述表型相关的几个过程有关。包括“免疫”，\"膜\",和“抗氧化活性”，特别是关于次级代谢产物。最重要的是,我们证明，FpVelB通过影响脱氧雪腐镰刀菌烯醇的产生和调节PKS11基因的表达来调节病原体的毒力。总之,FpVelB对植物生长至关重要，无性发育,和非生物应激反应，并且对于通过假草的次级代谢产生完全毒力至关重要。
