This review analyzes the occurrence and co-exposure of aflatoxins and fumonisins in conventional and organic corn, and compares the vulnerability to contamination of both. The risks of fungal contamination in corn are real, mainly by the genera Aspergillus and Fusarium, producers of aflatoxins and fumonisins, respectively. Aflatoxins, especially AFB1, are related to a high incidence of liver cancer, and the International Agency Research of Cancer (IARC) classified them in group 1A \'carcinogenic to humans\'. The occurrence in conventional corn is reported in many countries, including at higher levels than those established by legislation. IARC classified fumonisins in group 2B \'possibly carcinogenic to humans\' due to their link with incidence of esophageal cancer. However, comparing corn and organic and conventional by-products from different regions, different results are observed. The co-occurrence of both mycotoxins is a worldwide problem; nevertheless, there is little data on the comparison of the co-exposure of these mycotoxins in corn and derivatives between both systems. It was found that the agricultural system is not a decisive factor in the final contamination, indicating the necessity of effective strategies to reduce contamination and co-exposure at levels that do not pose health risks.

Corn silk (Zea mays L.) is the stigma of an annual gramineous plant named corn, which is distributed in many regions worldwide and has a long history of medicinal use. In recent years, with the sustainable development of traditional Chinese medicine, studies of corn silk based on modern technologies, such as GC-MS, LC-MS, and other analytical means, have offered more comprehensive analyses. Phytochemistry studies have shown that the main bioactive components in corn silk include flavonoids, polyphenols, phenolic acids, fatty acids, and terpenoids. Pharmacological studies have shown that corn silk extract has various pharmacological effects, such as reducing blood lipids, lowering blood pressure, regulating blood sugar levels, anti-inflammatory effects, and anti-oxidation effects. In this paper, the related research on corn silk from the past few years is summarized to provide a theoretical reference for the further development and utilization of corn silk.

In this study, hotspot regions, QTL clusters, and candidate genes for eight ear-related traits of maize (ear length, ear diameter, kernel row number, kernel number per row, kernel length, kernel width, kernel thickness, and 100-kernel weight) were summarized and analyzed over the past three decades. This review aims to (1) comprehensively summarize and analyze previous studies on QTLs associated with these eight ear-related traits and identify hotspot bin regions located on maize chromosomes and key candidate genes associated with the ear-related traits and (2) compile major and stable QTLs and QTL clusters from various mapping populations and mapping methods and techniques providing valuable insights for fine mapping, gene cloning, and breeding for high-yield and high-quality maize. Previous research has demonstrated that QTLs for ear-related traits are distributed across all ten chromosomes in maize, and the phenotypic variation explained by a single QTL ranged from 0.40% to 36.76%. In total, 23 QTL hotspot bins for ear-related traits were identified across all ten chromosomes. The most prominent hotspot region is bin 4.08 on chromosome 4 with 15 QTLs related to eight ear-related traits. Additionally, this study identified 48 candidate genes associated with ear-related traits. Out of these, five have been cloned and validated, while twenty-eight candidate genes located in the QTL hotspots were defined by this study. This review offers a deeper understanding of the advancements in QTL mapping and the identification of key candidates associated with eight ear-related traits. These insights will undoubtedly assist maize breeders in formulating strategies to develop higher-yield maize varieties, contributing to global food security.

Edge-of-field management practices that reduce nutrient pollution from tile drainage while contributing habitat and other ecosystem services are needed to enhance agricultural systems in the US Corn Belt Region. In this review, we identified edge-of-field and catchment scale agricultural conservation practices for intercepting and treating tile drainage. The reviewed conservation practices were (1) controlled drainage, also known as drainage water management (USDA-NRCS Code 554); (2) drainage water recycling (USDA-NRCS Code 447); (3) denitrifying bioreactors (USDA-NRCS Code 605); (4) saturated buffers (USDA-NRCS Code 604); and (5) constructed or restored wetlands designed for water quality improvement (USDA-NRCS Code 656) herein referred to as water quality wetlands. We examined 119 studies that had information on one or more of the following ecosystem services: water retention, water quality improvement (e.g., nitrate, phosphate, sediment, or pesticide retention), wetland habitat (for birds, aquatic organisms, and pollinators), crop yield improvement, and other benefits (e.g., recreation, education, aesthetic appreciation, greenhouse gas retention). We found the five edge-of-field practices were all effective at removing nitrate with varying degrees of other potential benefits and disservices (e.g., greenhouse gas production). Drainage water recycling and water quality wetlands have the potential to provide the most co-benefits as they provide surface water systems for capturing surface flows in addition to tile drainage while also potentially providing habitat and recreation opportunities. However, the following research needs are identified: 1) the disservices and benefits associated with drainage water recycling have not been adequately evaluated; 2) surface flow dynamics are understudied across all reviewed management practices; 3) a complete accounting of phosphorus species and flow pathways for all management practices is needed; 4) field evaluations of the habitat benefit of all management practices are needed; and 5) greenhouse gas dynamics are understudied across all management practices. While all management practices are expected to reduce nitrate loads, addressing these knowledge gaps will help inform holistic management decisions for diverse stakeholders across the US Corn Belt.

In many parts of the world, chemical pesticides are the primary method of pest control in maize (Zea mays L.) crops. Concerns about the negative consequences of chemical pesticide use on people\'s health and the environment, as well as the emergence of insecticide resistance, have accelerated attempts to discover alternatives that are effective, low-risk, and cost-effective. Maize-legume intercropping systems are known to have multiple benefits to agroecosystem functioning, including pest regulation. This review focuses on the influence of maize-legume intercropping systems on insect diversity and abundance as a mechanism for insect pest regulation in maize crops. First, this review combines knowledge of maize-legume intercrops, with a particular emphasis on the mechanism by which this practice attracts beneficial insects (e.g., predators, parasitoids) to reduce pest damage in intercropping systems. In addition, the pairings of specific legume species with the greatest potential to attract more beneficial insects and therefore reduce maize pests are also discussed. Finally, future research needs are also recommended. Findings are reviewed in the context of looking for long-term management strategies that can increase the adoption of integrated pest management programs in maize-based production systems.

Ischemic stroke in rodents is usually induced by intraluminal middle cerebral artery occlusion (MCAO) via the common carotid artery plugging filament invented by Koizumi et al. (MCAO-KM), or the external carotid artery plugging filament created by Zea Longa et al. (MCAO-LG). A systematic review of the distinctions between them is currently lacking. Here, we performed a meta-analysis in terms of model establishment, cerebral blood flow (CBF), and cerebral ischemia-reperfusion injury (CIRI) between them, Weighted Mean Differences and Standardized Mean Difference were used to analyze the combined effects, Cochrane\'s Q test and the I2 statistic were applied to determine heterogeneity, sensitivity analysis and subgroup analysis were performed to explore the source of heterogeneity. Literature mining suggests that MCAO-KM brings shorter operation time (p = 0.007), higher probability of plugging filament (p < 0.001) and molding establishment (p = 0.006), lower possibility of subarachnoid hemorrhage (p = 0.02), larger infarct volume (p = 0.003), severer brain edema (p = 0.002), and neurological deficits (p = 0.03). Nevertheless, MCAO-LG shows a more adequate CBF after ischemia-reperfusion (p < 0.001), a higher model survival rate (p = 0.02), and a greater infarct rate (p = 0.007). In conclusion, the MCAO-KM method is simple to operate with a high modeling success rate, and is suitable for the study of brain edema under long-term hypoperfusion, while the MCAO-LG method is highly challenging for novices, and is suitable for the study of CIRI caused by complete ischemia-reperfusion. These findings are expected to benefit the selection of intraluminal filament MCAO models before undertaking ischemic stroke preclinical effectiveness trials.

Zea mays (Z. mays) is one of the main cereal crops in the world, and it\'s by-products have exhibited medicinal properties to explore. This article intends to review the chemical compositions and pharmacological activities of by-products of Z. mays (corn silks, roots, bract, stems, bran, and leaves) which support the therapeutic potential in the treatment of different diseases, with emphasis on the natural occurring compounds and detailed pharmacological developments. Based on this review, 231 natural compounds are presented. Among them, flavonoids, terpenes, phenylpropanoids, and alkaloids are the most frequently reported. The by-products of Z. mays possess diuretic effects, hepatoprotective, anti-diabetic, antioxidant, neuroprotective, anti-inflammatory, anti-cancer, plant protection activity, and other activities. This article reviewed the phytochemistry and pharmacological activities of Z. mays for comprehensive quality control and the safety and effectiveness to enhance future application.

Temperature is a key factor in regulating and controlling several ecological processes. As there is a feedback relationship between many biogeochemical processes and climate change, their response to temperature changes is particularly important. Previously, a large volume of literature has extensively explored the impact of rising air temperature on shoot growth and maize yield, from enzymatic responses within the leaf to grain yield. As the global temperature continues to increase and the frequency, duration, and/or intensity of heat wave events increases, the soil temperature of the tilth is likely to rise sharply. As one of the most widely planted food crops in the world, maize may be subjected to additional soil temperature pressure. However, as a nutrient organ in direct contact with soil, the root plays a key role in adapting the whole plant to excessive soil temperature. Little research has been done on the effect of the soil microenvironment induced by higher soil temperature on maize root growth and root to shoot communication regulation. Therefore, this review summarizes (1) the effects of excessive soil temperature on the soil microenvironment, including soil respiration, microbial community composition, carbon mineralization, and enzyme activity; (2) the negative response of absorption of water and nutrients by roots and maize root-shoot growth to excessive soil temperature; and (3) potential cultivation strategies to improve maize yield, including improving tillage methods, adding biochar amendments, applying organic fertilizers, optimizing irrigation, and farmland mulching.

BACKGROUND: Maize lethal necrosis (MLN) disease is a significant constraint for maize producers in sub-Saharan Africa (SSA). The disease decimates the maize crop, in some cases, causing total crop failure with far-reaching impacts on regional food security.
RESULTS: In this review, we analyze the impacts of MLN in Africa, finding that resource-poor farmers and consumers are the most vulnerable populations. We examine the molecular mechanism of MLN virus transmission, role of vectors and host plant resistance identifying a range of potential opportunities for genetic and phytosanitary interventions to control MLN. We discuss the likely exacerbating effects of climate change on the MLN menace and describe a sobering example of negative genetic association between tolerance to heat/drought and susceptibility to viral infection. We also review role of microRNAs in host plant response to MLN causing viruses as well as heat/drought stress that can be carefully engineered to develop resistant varieties using novel molecular techniques.
CONCLUSIONS: With the dual drivers of increased crop loss due to MLN and increased demand of maize for food, the development and deployment of simple and safe technologies, like resistant cultivars developed through accelerated breeding or emerging gene editing technologies, will have substantial positive impact on livelihoods in the region. We have summarized the available genetic resources and identified a few large-effect QTLs that can be further exploited to accelerate conversion of existing farmer-preferred varieties into resistant cultivars.

Carbohydrates are the primary energy source for lactating dairy cows, and dairy diets are usually formulated for certain concentrations of forage neutral detergent fiber (NDF) and starch due to their direct effects on dry matter intake and milk production. Forage NDF exerts greater filling effects in the rumen than other dietary components and can limit maximum voluntary feed intake of lactating dairy cows. Since an analytical method for NDF was developed more than a half century ago, it has been used widely to characterize forages and diets for dairy cows. However, because NDF is a chemical measurement varying in its digestibility, in vitro digestibility measurements were developed as a biological approach to assess forage quality. Research efforts over the last several decades led to the development of forage cultivars or hybrids with enhanced in vitro NDF digestibility, such as brown midrib, and management practices considering differences in NDF digestibility of forages. In addition, in vitro NDF digestibility and undigested NDF are commonly measured in commercial labs, and estimated rates of digestion are used in dynamic models in an effort to improve the accuracy and precision of diet formulation. Starch digestion in the rumen also varies among starch sources, being affected by grain type, extent of processing, and conservation method. The site and rate of starch digestion affect dry matter intake and nutrient partitioning in dairy cows by modifying temporal supply of fuel. In addition, dietary starch content and its fermentability can affect digestion rates of starch itself and NDF in the rumen. Previous research has increased our understanding of dietary carbohydrates, but its application for diet formulations requires integrated approaches accounting for factors affecting the filling effects of forage NDF, starch digestion, and temporal fuel supply.