■FAR1/FHY3转录因子来源于转座酶,在光信号转导中起重要作用,成长和发展,并通过调节下游基因表达来响应胁迫。尽管许多FAR1/FHY3成员已在各种物种中被鉴定,玉米中的FAR1/FHY3基因没有得到很好的表征,它们在干旱中的功能也是未知的。
■使用PlantTFDB鉴定了玉米基因组中的FAR1/FHY3家族,普法姆,聪明,和NCBI-CDD网站。为了研究FAR1基因在玉米中的进化和功能,蛋白质序列的信息,染色体定位,亚细胞定位,保守的图案,通过生物信息学分析了进化关系和组织表达模式,通过实时定量聚合酶链反应(qRT-PCR)检测干旱胁迫下的表达模式。
■玉米基因组中共有24个ZmFAR成员,可以分为五个亚科,亚家族之间的蛋白质和基因结构差异很大。ZmFAR的启动子区域包含丰富的非生物胁迫响应性和激素响应性顺式元件。其中,干旱响应的顺式元素相当丰富。ZmFAR在检测到的所有组织中均有表达,但表达水平差异很大。ZmFAR的表达在原代根中大部分下调,精根,侧根,和缺水下的中胚轴。PEG模拟干旱胁迫后,大多数ZmFAR在根中下调。
■我们对玉米中的FAR1/FHY3基因进行了全基因组和系统的鉴定。干旱胁迫后,大多数ZmFAR在根系中下调。这些结果表明,FAR1/FHY3转录因子在干旱胁迫响应中具有重要作用。为进一步分析ZmFAR对干旱胁迫的响应功能奠定基础。
UNASSIGNED: FAR1/FHY3 transcription factors are derived from transposase, which play important roles in light signal transduction, growth and development, and response to stress by regulating downstream gene expression. Although many FAR1/FHY3 members have been identified in various species, the FAR1/FHY3 genes in maize are not well characterized and their function in drought are unknown.
UNASSIGNED: The FAR1/FHY3 family in the maize genome was identified using PlantTFDB, Pfam, Smart, and NCBI-CDD websites. In order to investigate the evolution and functions of FAR1 genes in maize, the information of protein sequences, chromosome localization, subcellular localization, conserved motifs, evolutionary relationships and tissue expression patterns were analyzed by bioinformatics, and the expression patterns under drought stress were detected by quantitative real-time polymerase chain reaction (qRT-PCR).
UNASSIGNED: A total of 24 ZmFAR members in maize genome, which can be divided into five subfamilies, with large differences in protein and gene structures among subfamilies. The promoter regions of ZmFARs contain abundant abiotic stress-responsive and hormone-respovensive cis-elements. Among them, drought-responsive cis-elements are quite abundant. ZmFARs were expressed in all tissues detected, but the expression level varies widely. The expression of ZmFARs were mostly down-regulated in primary roots, seminal roots, lateral roots, and mesocotyls under water deficit. Most ZmFARs were down-regulated in root after PEG-simulated drought stress.
UNASSIGNED: We performed a genome-wide and systematic identification of FAR1/FHY3 genes in maize. And most ZmFARs were down-regulated in root after drought stress. These results indicate that FAR1/FHY3 transcription factors have important roles in drought stress response, which can lay a foundation for further analysis of the functions of ZmFARs in response to drought stress.