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Abstract：
Ischemic stroke in rodents is usually induced by intraluminal middle cerebral artery occlusion (MCAO) via the common carotid artery plugging filament invented by Koizumi et al. (MCAO-KM), or the external carotid artery plugging filament created by Zea Longa et al. (MCAO-LG). A systematic review of the distinctions between them is currently lacking. Here, we performed a meta-analysis in terms of model establishment, cerebral blood flow (CBF), and cerebral ischemia-reperfusion injury (CIRI) between them, Weighted Mean Differences and Standardized Mean Difference were used to analyze the combined effects, Cochrane\'s Q test and the I2 statistic were applied to determine heterogeneity, sensitivity analysis and subgroup analysis were performed to explore the source of heterogeneity. Literature mining suggests that MCAO-KM brings shorter operation time (p = 0.007), higher probability of plugging filament (p < 0.001) and molding establishment (p = 0.006), lower possibility of subarachnoid hemorrhage (p = 0.02), larger infarct volume (p = 0.003), severer brain edema (p = 0.002), and neurological deficits (p = 0.03). Nevertheless, MCAO-LG shows a more adequate CBF after ischemia-reperfusion (p < 0.001), a higher model survival rate (p = 0.02), and a greater infarct rate (p = 0.007). In conclusion, the MCAO-KM method is simple to operate with a high modeling success rate, and is suitable for the study of brain edema under long-term hypoperfusion, while the MCAO-LG method is highly challenging for novices, and is suitable for the study of CIRI caused by complete ischemia-reperfusion. These findings are expected to benefit the selection of intraluminal filament MCAO models before undertaking ischemic stroke preclinical effectiveness trials.
摘要：
啮齿动物的缺血性中风通常是由Koizumi等人发明的颈总动脉阻塞丝引起的管腔内大脑中动脉阻塞（MCAO）引起的。(MCAO-KM),或ZeaLonga等人创建的颈外动脉堵塞丝。（MCAO-LG）。目前缺乏对它们之间区别的系统审查。这里,我们在模型建立方面进行了荟萃分析，脑血流量（CBF），以及它们之间的脑缺血再灌注损伤（CIRI），加权平均差和标准化平均差用于分析综合效应，应用Cochrane的Q检验和I2统计量确定异质性，进行敏感性分析和亚组分析以探索异质性的来源。文献挖掘表明，MCAO-KM带来了更短的操作时间(p=0.007)，更高的可能性堵塞细丝(p<0.001)和成型建立(p=0.006)，蛛网膜下腔出血的可能性较低（p=0.02），梗死体积较大（p=0.003），严重的脑水肿（p=0.002），和神经功能缺损（p=0.03）。然而，MCAO-LG在缺血再灌注后显示出更充足的CBF(p<0.001),较高的模型生存率(p=0.02)，和更大的梗塞率（p=0.007）。总之,MCAO-KM方法操作简单，建模成功率高，适用于长期低灌注脑水肿的研究，虽然MCAO-LG方法对新手来说极具挑战性，适用于完全缺血再灌注引起的CIRI的研究。这些发现有望在进行缺血性卒中临床前有效性试验之前有利于选择管腔内细丝MCAO模型。
