传染病是全球水产养殖业和保护计划损失的主要原因。同时,传染病对孵化场饲养和释放到自然栖息地进行保护的鱼类构成重大风险,包括五大湖湖st鱼(AcipenserFulvescens,即,GL-LST)。最近,同种疱疹病毒(湖鱼疱疹病毒2型,即,在两个成年GL-LST群体中检测到LSHV-2)能够在成年和少年GL-LST中诱导疾病和/或死亡。开始开发疾病预防和/或控制方法,体外实验旨在确定LSHV-2对孵化场和水产养殖设施中常用的消毒剂(Virkon®-Aquatic:过氧单硫酸钾;Ovadine®:聚乙烯吡咯烷酮碘复合物;和Perox-Aid®:过氧化氢)的敏感性。将培养的LSHV-2暴露于两种浓度的每种消毒剂(Virkon®-Aquatic:0.5%和1%;Ovadine®:50和100ppm;Perox-Aid®:500和1000ppm),一式两份,持续1、10和30分钟。曝光后,消毒剂被中和,在白st鱼×湖st鱼杂交细胞系(WSxLS)上经过14天的潜伏期后,通过比较有和没有消毒剂暴露的病毒的50%组织培养感染剂量(TCID50/mL)来计算减少百分比.当暴露于Perox-Aid®时,LSHV-2%的降低范围为58.7%至99.5%。当暴露于Ovadine®时,减少的百分比从99.4%到100%不等。最后,当暴露于Virkon®-Aquatic时,对于两种浓度和所有时间点,减少百分比为100%。本文的结果提供了Virkon®-Aquatic和Ovadine®对LSHV-2都是杀病毒的证据,并且可以代表在野外设置下降低病毒传播风险的手段。
Infectious diseases are a leading cause of losses in the aquaculture industry and conservation programs globally. Simultaneously, infectious diseases pose a substantial risk to fish being hatchery-reared and released into natural habitats for conservation purposes, including the Great Lakes lake sturgeon (Acipenser fulvescens, i.e., GL-LST). Recently, an alloherpesvirus (lake sturgeon herpesvirus 2, i.e., LSHV-2) capable of inducing disease and/or mortality in adult and juvenile GL-LSTs was detected in two adult GL-LST populations. To begin developing disease prevention and/or control methods, in vitro experiments were designed to determine the susceptibility of LSHV-2 to disinfectants commonly used in hatchery and aquaculture facilities (Virkon®-Aquatic: potassium peroxymonosulfate; Ovadine®: polyvinylpyrrolidone iodine complex; and Perox-Aid®: hydrogen peroxide). Cultured LSHV-2 was exposed to each disinfectant at two concentrations (Virkon®-Aquatic: 0.5% and 1%; Ovadine®: 50 and 100 ppm; and Perox-Aid®: 500 and 1000 ppm) in duplicate for durations of 1, 10, and 30 min. Following exposure, the disinfectant was neutralized, and after a 14-day incubation period on a white sturgeon × lake sturgeon hybrid cell line (WSxLS), percent reduction was calculated by comparing the 50% tissue culture infectious doses (TCID50/mL) of the virus with and without disinfectant exposure. When exposed to Perox-Aid®, LSHV-2 percent reduction ranged from 58.7% to 99.5%. When exposed to Ovadine®, the percent reduction ranged from 99.4% to 100%. Lastly, the percent reduction when exposed to Virkon®-Aquatic was 100% for both concentrations and all timepoints. The results herein provide evidence that both Virkon®-Aquatic and Ovadine® are virucidal to LSHV-2 and may represent a means to reduce virus transmission risk under field settings.