Abstract：
Electrochemical profiling of formaldehyde-inactivated poliovirus particles demonstrated a relationship between the D-antigen concentration and the intensity of the maximum amplitude currents of the poliovirus samples. The resultant signal was therefore identified as electrochemical oxidation of the surface proteins of the poliovirus. Using registration of electrooxidation of amino acid residues of the capsid proteins, a comparative electrochemical analysis of poliovirus particles inactivated by electrons accelerated with doses of 5 kGy, 10 kGy, 15 kGy, 25 kGy, 30 kGy at room temperature was carried out. An increase in the radiation dose was accompanied by an increase in electrooxidation signals. A significant increase in the signals of electrooxidation of poliovirus capsid proteins was detected upon irradiation at doses of 15-30 kGy. The data obtained suggest that the change in the profile and increase in the electrooxidation signals of poliovirus capsid proteins are associated with an increase in the degree of structural reorganization of surface proteins and insufficient preservation of the D-antigen under these conditions of poliovirus inactivation.
摘要：
甲醛灭活的脊髓灰质炎病毒颗粒的电化学分析表明，D抗原浓度与脊髓灰质炎病毒样品的最大振幅电流强度之间存在关系。因此,所得信号被鉴定为脊髓灰质炎病毒表面蛋白的电化学氧化。使用衣壳蛋白氨基酸残基的电氧化注册，通过5kGy剂量加速的电子灭活的脊髓灰质炎病毒颗粒的比较电化学分析，10kGy,15kGy,25kGy,在室温下进行30kGy。辐射剂量的增加伴随着电氧化信号的增加。在15-30kGy剂量的照射下，检测到脊髓灰质炎病毒衣壳蛋白的电氧化信号显着增加。获得的数据表明，在脊髓灰质炎病毒灭活条件下，脊髓灰质炎病毒衣壳蛋白的谱变化和电氧化信号增加与表面蛋白结构重组程度的增加和D-抗原保存不足有关。
