关键词： AE ANOVA BDI DMT1+IRE DMT1−IRE DTPA E1 E2 Exercise Fpn HC Hippocampus IRE IRP1 IRP2 Iron metabolism L-NAME MDA N(ω)-nitro-L-arginine methyl ester NHI NO NOS NOx Nitric oxide ROS Redox-active iron S1 S2 SEM Storage iron TCA TfR1 aerobic exercise bleomycin-detectable iron diethylene triamine pentacetate acid divalent metal transporter 1 with iron responsive element divalent metal transporter 1 without iron responsive element eNOS endothelial nitric oxide synthase exercise group exercise+L-NAME group ferroportin 1 hippocampus iNOS inducible nitric oxide synthase iron regulatory protein 1 iron regulatory protein 2 iron responsive element malondialdehyde nNOS neuronal nitric oxide synthase nitric oxide nitric oxide synthase nitrite plus nitrate nonheme iron reactive oxygen species sedentary group sedentary+L-NAME group standard error of mean transferrin receptor 1 trichloroacetic acid two-way analysis of variance

Mesh ： Animals Antioxidants / chemistry Bleomycin / chemistry Cation Transport Proteins / chemistry metabolism Female Hippocampus / metabolism Hydrogen Peroxide / chemistry Iron / chemistry NG-Nitroarginine Methyl Ester / chemistry Nitric Oxide / chemistry Oxidation-Reduction Oxidative Stress Physical Conditioning, Animal Random Allocation Rats Rats, Sprague-Dawley Receptors, Transferrin / metabolism Risk

来  源：   DOI：10.1016/j.niox.2013.10.009

Abstract：
Adult hippocampus is highly vulnerable to iron-induced oxidative stress. Aerobic exercise has been proposed to reduce oxidative stress but the findings in the hippocampus are conflicting. This study aimed to observe the changes of redox-active iron and concomitant regulation of cellular iron homeostasis in the hippocampus by aerobic exercise, and possible regulatory effect of nitric oxide (NO). A randomized controlled study was designed in the rats with swimming exercise treatment (for 3 months) and/or an unselective inhibitor of NO synthase (NOS) (L-NAME) treatment. The results from the bleomycin-detectable iron assay showed additional redox-active iron in the hippocampus by exercise treatment. The results from nonheme iron content assay, combined with the redox-active iron content, showed increased storage iron content by exercise treatment. NOx (nitrate plus nitrite) assay showed increased NOx content by exercise treatment. The results from the Western blot assay showed decreased ferroportin expression, no changes of TfR1 and DMT1 expressions, increased IRP1 and IRP2 expression, increased expressions of eNOS and nNOS rather than iNOS. In these effects of exercise treatment, the increased redox-active iron content, storage iron content, IRP1 and IRP2 expressions were completely reversed by L-NAME treatment, and decreased ferroportin expression was in part reversed by L-NAME. L-NAME treatment completely inhibited increased NOx and both eNOS and nNOS expression in the hippocampus. Our findings suggest that aerobic exercise could increase the redox-active iron in the hippocampus, indicating an increase in the capacity to generate hydroxyl radicals through the Fenton reactions, and aerobic exercise-induced iron accumulation in the hippocampus might mainly result from the role of the endogenous NO.
摘要：
成人海马极易受到铁诱导的氧化应激的影响。有氧运动已被提议减少氧化应激，但在海马中的发现是相互矛盾的。本研究旨在观察有氧运动对海马中氧化还原活性铁的变化以及对细胞铁稳态的调节。一氧化氮（NO）的可能调节作用。在进行游泳运动治疗（3个月）和/或NO合酶（NOS）非选择性抑制剂（L-NAME）治疗的大鼠中设计了一项随机对照研究。博来霉素可检测的铁测定法的结果表明，通过运动处理，海马中增加了氧化还原活性铁。非血红素铁含量测定的结果，结合氧化还原活性铁含量，显示通过运动处理增加了储存铁含量。NOx（硝酸盐加亚硝酸盐）测定显示，运动处理后NOx含量增加。Westernblot检测结果显示，亚铁转运蛋白表达减少，TfR1和DMT1表达式没有变化，IRP1和IRP2表达增加，eNOS和nNOS而不是iNOS的表达增加。在运动治疗的这些效果中，氧化还原活性铁含量增加，储存铁含量，IRP1和IRP2表达通过L-NAME处理完全逆转,减少的铁转运蛋白表达被L-NAME部分逆转。L-NAME处理完全抑制了海马中NOx的增加以及eNOS和nNOS的表达。我们的研究结果表明，有氧运动可以增加海马中的氧化还原活性铁，表明通过芬顿反应产生羟基自由基的能力增加，有氧运动引起的海马铁积累可能主要是由于内源性NO的作用。
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