SEM

SEM
  • 文章类型: Journal Article
    不同的挤压条件对无壳大麦-绿豆(70:30)挤压零食的功能特性的影响进行了研究,使用响应面法和饲料湿度(FM),机筒温度(BT),和螺杆速度(SS)作为过程变量。结果表明,不同的挤出条件对功能特性有重大影响。挤出零食的堆积密度(BD)范围为0.24至0.42g/cm3,表明较低的FM和较高的BT导致较低的BD,而随着FM的增加而增加。SS,和BT。挤出零食的膨胀比(ER)介于2.03和2.33之间,表明BT和SS具有理想的积极作用,而增加FM导致ER降低。BT和SS的增加对吸水率有负面影响,而调频显示出积极的影响,其范围在4.21和4.82g/g之间。BT和SS描述了对水溶性指数的积极影响,介于9.01%至13.45%之间,较高的SS和BT导致淀粉降解和溶解度增加,表明更好的消化率。挤压零食的硬度范围为32.56至66.88牛顿(N),显示增加FM增加硬度,而较高的SS和BT导致硬度降低。扫描电子显微镜(SEM)分析显示,与非挤出面粉相比,挤出物的结构变化,表明淀粉糊化和孔隙形成受不同加工参数的影响。在傅里叶变换红外光谱(FT-IR)中观察到吸收带的变化,提示淀粉和蛋白质的结构变化。了解挤出参数对产品特性的影响可以帮助定制生产以满足消费者的喜好,并开发具有改善营养质量的功能性零食。
    The effect of varying extrusion conditions on the functional properties of hulless barley-mung bean (70:30) extruded snacks was investigated using response surface methodology with feed moisture (FM), barrel temperature (BT), and screw speed (SS) as process variables. Results revealed significant impacts on functional characteristics with varying extrusion conditions. Bulk density (BD) of extruded snacks ranged from 0.24 to 0.42 g/cm3, showing that lower FM and higher BT results in lower BD while it increased with increasing FM, SS, and BT. The expansion ratio (ER) of extruded snacks ranged between 2.03 and 2.33, showing BT and SS had a desirable positive effect, whereas increasing FM led to decreased ER. Increasing BT and SS depicted a negative effect on water absorption index, whereas FM showed positive effect, which ranged between 4.21 and 4.82 g/g. A positive effect on water solubility index was depicted by BT and SS, which ranges between 9.01% and 13.45%, as higher SS and BT led to starch degradation and increased solubility suggesting better digestibility. The hardness of extruded snacks ranged from 32.56 to 66.88 Newton (N), showing increasing FM increased hardness, whereas higher SS and BT resulted in lowering the hardness. Scanning electronic microscope (SEM) analysis revealed structural changes in extrudates in comparison with nonextruded flour, indicating starch gelatinization and pore formation affected by varying processing parameters. Shifts in absorption bands were observed in Fourier transform infrared spectroscopy (FT-IR), suggesting structural changes in starch and protein. Understanding the effects of extrusion parameters on product properties can help tailored production to meet consumers\' preferences and the development of functional snacks with improved nutritional quality.
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  • 文章类型: Journal Article
    包裹在Cladoceranepippia中的滞育胚胎是有性生殖的结果,并增加了遗传多样性。它们也是物种绕过恶劣环境条件并在空间和时间上分散的重要手段。一旦被释放,ephippia通常沉入底栖动物并留在那里直到孵化。使用Sars方法(孵化沉积物以识别剑角幼虫),可以评估产卵库的生物多样性。然而,即使样品在各种条件下孵育,不可能保证所有人都孵化了。很少有钥匙可以通过仅使用海马形态来促进对剑角的识别。我们的目标是分析一些来自墨西哥的Cladoceranephippia,开发一种使用易于识别的特征来识别它们的方法。来自阿瓜斯卡连特斯(墨西哥)11属水域的23种锁骨物种的Ephippia(Alona,Biapertura,Ceriodapnia,Chydorus,水蚤,Dunhevedia,伊利克伦,Macrothrix,莫伊纳,Pleuroxus,和Simocephalus)进行了分析。在我们的分析中,选择了六个形态特征,这些形态特征允许在物种(-组)水平上鉴定阿菲皮亚。结果表明,通过适当的功能目录,可以识别出一些epippia。
    Diapausing embryos encased within cladoceran ephippia result from sexual reproduction and increase genetic diversity. They are also important means by which species bypass harsh environmental conditions and disperse in space and time. Once released, ephippia usually sink to the benthos and remain there until hatching. Using the Sars\' method (incubating sediments to identify cladoceran hatchlings), ephippial egg bank biodiversity can be evaluated. Yet, even when samples are incubated under a variety of conditions, it is not possible to warrant that all have hatched. Few keys are available that facilitate the identification of cladocerans by using only ephippial morphology. Our goal was to analyze some cladoceran ephippia from Mexico, to develop a means to identify them using easily recognizable characteristics. Ephippia of 23 cladoceran species from waters in Aguascalientes (México) in 11 genera (Alona, Biapertura, Ceriodaphnia, Chydorus, Daphnia, Dunhevedia, Ilyocryptus, Macrothrix, Moina, Pleuroxus, and Simocephalus) were analyzed. In our analysis six morphological features were selected that permitted the identification of ephippia to species(-group) level. The results demonstrate that with a proper catalog of features, some ephippia can be identified.
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  • 文章类型: Journal Article
    干bonito(Katsuobushi)在传统的烟雾干燥后表现出独特的均匀“玻璃状”质地。在这里,我们开发了一种新的处理方法,用于干bonito,并阐明了在纹理方面将松弛肌肉转化为“玻璃样”纹理的机制,微观结构,和蛋白质特性。我们的发现表明,热诱导后蛋白质的展开和聚集是塑造骨骼肌肉“玻璃样”质地的关键因素。在处理过程中,肌原纤维聚集,最初交替的粗细细丝横向收缩并排成一条直线,和蛋白质交联增加。二级结构分析显示,不稳定的β转角含量从26.28%降低到15.06%。此外,观察到SS键的含量增加,构象从g-g-t变为稳定的g-g-g构象,增强蛋白质构象稳定性。一起来看,我们的研究结果为理解干bonito中均匀的“玻璃样”纹理的形成机理提供了理论基础。
    Dried-bonito (Katsuobushi) exhibits a unique uniform \"glass-like\" texture after traditional smoke-drying. Herein, we developed a novel processing method for dried-bonito and elucidated the mechanism of transformation of loose muscle into a \"glass-like\" texture in terms of texture, microstructure, and protein properties. Our findings showed that the unfolding and aggregation of proteins after thermal induction was a key factor in shaping the \"glass-like\" texture in bonito muscle. During processing, myofibrils aggregated, the originally alternating thick and thin filaments contracted laterally and aligned into a straight line, and protein cross-linking increased. Secondary structural analysis revealed a reduction in unstable β-turn content from 26.28% to 15.06%. Additionally, an increase in the content of SS bonds was observed, and the conformation changed from g-g-t to a stable g-g-g conformation, enhanced protein conformational stability. Taken together, our findings provide a theoretical basis for understanding the mechanism of formation of the uniform \"glass-like\" texture in dried-bonito.
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  • 文章类型: Journal Article
    该研究旨在对海内酰脲和硫内酰脲化合物对成熟的曼氏血吸虫进行体外生物学评估,使用计算方法评估其细胞毒性作用并预测其药代动力学参数。所述化合物显示低的体外细胞毒性并且不被认为是溶血的。用浓度范围为200至6.25μM的所有化合物测试了对成虫S.mansoni蠕虫的抗寄生虫活性。化合物SC01、SC02和SC03表现出低活性。化合物SC04、SC05、SC06和SC07在100和200μM的浓度下孵育24小时内引起100%死亡率。硫内酰脲SC04表现出最高的活性,在50μM的浓度和28μM的IC50孵育24小时后导致100%死亡率。在超微结构分析(SEM)中,化合物SC04(200µM)诱导外皮变化,外皮水泡的形成,以及结节和针状体的破坏。因此,SC04化合物显示出有望作为抗曼氏芽孢杆菌的抗寄生虫药。
    The study aimed to conduct in vitro biological assessments of hydantoin and thiohydantoin compounds against mature Schistosoma mansoni worms, evaluate their cytotoxic effects and predict their pharmacokinetic parameters using computational methods. The compounds showed low in vitro cytotoxicity and were not considered hemolytic. Antiparasitic activity against adult S. mansoni worms was tested with all compounds at concentrations ranging from 200 to 6.25μM. Compounds SC01, SC02, and SC03 exhibited low activity. Compounds SC04, SC05, SC06 and SC07 caused 100% mortality within 24h of incubation at a concentration of 100 and 200μM. Thiohydantoin SC04 exhibited the highest activity, resulting in 100% mortality after 24h of incubation at a concentration of 50μM and IC50 of 28µM. In the ultrastructural analysis (SEM), the compound SC04 (200µM) induced integumentary changes, formation of integumentary blisters, and destruction of tubercles and spicules. Therefore, the SC04 compound shows promise as an antiparasitic against S. mansoni.
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  • 文章类型: Journal Article
    在这项工作中,根据分子标记分析(线粒体COI,16SrDNA和核ITS2)和形态特征。我们的结果表明,在所研究的材料中至少存在五种遗传谱系,这些遗传谱系也可以通过其分段环状模式在形态上进行鉴定。针状棘的数量和形状,以及管状裂片的长度和形状。Orbiniellapetersenae的物种名称被分配给其中一个谱系,将其地理和垂直分布限制在冰岛和扬马延以北的深海地区,和三个谱系被描述为新物种(即,OrbiniellagriegiMeca&Budaeva,sp.11月。,OrbiniellamayhemiMeca&Budaeva,sp.11月。,和OrbiniellaaporiMeca&Budaeva,sp.11月。)将该属中已知物种的数量提高到25个。在我们的研究中,仅在格陵兰-冰岛-苏格兰山脊以北报告了三种深海Orbiniella物种,在山脊以南发现了一种深海物种。沿山脊和挪威架子上分布着一种浅水物种。
    In this work, the diversity of the genus Orbiniella in the Nordic Seas and the North Atlantic waters south of Iceland is studied based on the analyses of molecular markers (mitochondrial COI, 16S rDNA and nuclear ITS2) and morphological characters. Our results showed the presence of at least five genetic lineages in the studied material which could also be morphologically identified by their segmental annulation patterns, the number and the shape of acicular spines, and the length and the shape of pygidial lobes. The species name Orbiniellapetersenae is assigned to one of the lineages restricting its geographical and vertical distribution to the deep-sea areas north of Iceland and Jan Mayen, and three lineages are described as new species (i.e., Orbiniellagriegi Meca & Budaeva, sp. nov., Orbiniellamayhemi Meca & Budaeva, sp. nov., and Orbiniellaparapari Meca & Budaeva, sp. nov.) elevating the number of known species in the genus to 25. Three deep-sea species of Orbiniella in our study are reported only north of the Greenland-Iceland-Scotland Ridge, one deep-sea species found south of the ridge. A single shallow-water species is distributed along the ridge and on the Norwegian shelf.
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  • 文章类型: Journal Article
    这项研究是为了调查宏观解剖学,形态计量学,组织学,和成年雄性和雌性火鸡(Meleagrisgallapovo)的尿道腺的电子显微镜特征。
    这项研究是为了研究宏观解剖学,形态计量学,组织学,和成年雄性和雌性火鸡(Meleagrisgallopavo)的尿道腺的电子显微镜特征。本研究使用了13只成年火鸡(6只雄性和7只雌性)的阴性腺。确定位于最后一个尾椎背侧的腺体结构是心形的,由两个裂片和一个乳头系统组成。作为统计评估的结果,在叶片长度的参数上,性别之间存在显着差异,乳头宽度和乳头高度(p<.05)。此外,尿路脓性乳头指数(PUI)值差异有统计学意义(p<.05)。为确定腺体的组织学结构而采集的组织样品用苏木精和伊红染色,克罗斯曼的三倍,高碘酸希夫(PAS),淀粉酶,和orcein.组织学检查显示,腺体具有由结缔组织组成的囊包围的两瓣结构。确定腺体具有管状结构,上皮层由细胞层组成,作为发芽层,中间层,分泌层,从外围到中心的退化层。在扫描电子显微镜检查中,腺体的叶结构由从表面突出的不同形状的毛囊组成。观察到这些腺卵泡结构以不同的方式聚集在一起或大部分杂乱无章。作为研究的结果,确定火鸡尿道腺的形态和组织学结构与其他鸟类相似,但总体上表现出一些物种特异性和栖息地依赖性的差异。Arcan研究重点:在雄性和雌性火鸡中,尿道腺由两个裂片和一个乳头的系统组成。腺叶由结缔组织包围的毛囊组成,而卵泡通过卵泡间间隔相互连接。在形态测量中获得的结果揭示了雄性和雌性鸟类之间的统计差异。组织学检查显示高碘酸希夫(PAS)(+)在小管上皮细胞的基底膜中反应并分泌。在结缔组织和管间区域都发现了淋巴样细胞群落。该反应不是糖原诱导的事实是通过观察PAS-淀粉酶技术染色的结果而确定的。
    This study was carried out to investigate the macroanatomical, morphometric, histological, and electron microscopic characteristics of the uropygial gland in adult male and female turkeys (Meleagris gallapovo).
    This study was carried out to investigate the macroanatomical, morphometric, histological, and electron microscopic characteristics of the uropygial gland in adult male and female turkeys (Meleagris gallopavo). The glandula uropygialis of 13 adult turkeys (6 males and 7 females) were used for this study. It was determined that this glandular structure located dorsal to the last caudal vertebrae was heart‐shaped and consisted of two lobes and a papilla system. As a result of statistical evaluation, significant differences were found between the sexes in the parameters of lobe length, papilla width and papilla height (p < .05). Also, papilla uropygialis index (PUI) value had a statistically significant difference (p < .05). Tissue samples taken to determine the histological structure of the gland were stained with Hematoxylin & Eosin, Crossman\'s triple, periodic acid Schiff (PAS), diastase, and orcein. Histological examination revealed that the gland had a two‐lobed structure surrounded by a capsule composed of connective tissue. It was determined that the gland had a tubular structure and the epithelial layer consisted of cellular layers as a germinative layer, intermediate layer, secretory layer, and degenerative layer from the periphery to center. In the scanning electron microscope examination, the lobe structure of the gland consisted of different shaped follicles protruding from the surface. It was observed that these glandular follicle structures were gathered together in different ways or mostly disorganized. As a result of the study, it was determined that the morphological and histological structure of the uropygial gland in turkeys was similar to that of other bird species, but showed some species‐specific and habitat‐dependent differences in general. Arıcan RESEARCH HIGHLIGHTS: The uropygial gland consisted of a system of two lobes and one papilla in each of male and female turkeys. The glandular lobes consisted of follicles surrounded by connective tissue, while the follicles were connected to each other by interfollicular septae. The results obtained in morphometric measurements revealed statistical differences between male and female birds. Histological examination showed periodic acid Schiff (PAS) (+) reaction in the basal membranes of tubule epithelial cells and secretion. Lymphoid cell communities were found in both connective tissue and intertubular regions. The fact that the reaction was not glycogen‐induced was determined by the observation of PAS positivity as a result of staining with the PAS‐diastase technique.
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  • 文章类型: Journal Article
    本研究试图通过知识共享(KNS)来调查巴基斯坦高等教育机构(HEI)变革型领导者(TFL)与追随者成果创新工作行为(IWB)和任务绩效之间的动态联系。使用定量设计,采用了一种采用的结构来获得HEI领导者和员工行为的响应。通过SmartPLS通过结构方程建模(SEM)技术分析获得的信息。结果描述了大学变革型领导与员工之间的直接联系。创新工作行为以及任务绩效。结果进一步假设,在HEI的背景下,KNS调解了变革型领导与员工TSP之间的关系。令人惊讶的是,KNS无法明显成为中介变量,以加强巴基斯坦HEIs部门的变革型领导力与IWB员工之间的关系。除了增强高等教育领导力的理论理解,本文的结果认为,促进知识共享文化对于现有和未来的HEIs领导者来说都是宝贵的资产,以促进创新和创造力的文化。尽管最近的研究调查了KNS作为中介的作用,然而,本研究首次使用KNS作为IWB和任务绩效的同期干预变量。该研究还证实了社会交换机制在加强领导者成员连续体之间关系方面的理论基础。
    This research tries to investigate the dynamic link between higher education institution (HEIs) transformational leaders (TFL) and follower\'s outcome innovative work behavior (IWB) and Task Performance) through Knowledge sharing (KNS) in Pakistan. Using quantitative design an adopted construct was used to obtain response from HEIs leaders and employees behavior. The obtained information was analyzed through structural equation modeling (SEM) technique via Smart PLS. Results depict that direct link between University Transformational leadership and employees Innovative work behavior as well as Task Performance. The results further postulate that KNS mediate the relationship between Transformational leadership and employees TSP in the context of HEIs. Surprisingly, KNS could not evident to become a mediating variable to strengthen the relationship between transformational Leadership and employees IWB in the HEIs sector of Pakistan. In addition to enhancing the theoretical comprehension of higher education leadership, the outcomes of this article provide that promoting knowledge sharing culture is valuable asset for both existing and future HEIs leaders in order to promote the culture of innovation and creativity. Although recent studies investigate the role of KNS as a mediator, however the current study use KNS as contemporaneous intervening variable for IWB and Task Performance for the first time. The study also confirms theoretical underpinning of social exchange mechanism in strengthening the relationship between leader member\'s continuum.
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  • 文章类型: Journal Article
    本文介绍了经过化学处理的非合金结构钢和合金(马氏体)钢表面几何结构的表征结果。磷化前,对样品进行热处理。研究了样品的表面和横截面。使用扫描电子显微镜(SEM)进行了详细的研究,XRD,金相显微镜,化学成分分析和分形分析。表面几何形状的特征包括圆度等参数,圆度,坚固性,费雷特的直径,分水岭直径,分形维数和角频率,通过对SEM图像的数值处理进行计算。
    The article presents the results of the characterization of the geometric structure of the surface of unalloyed structural steel and alloyed (martensitic) steel subjected to chemical processing. Prior to phosphating, the samples were heat-treated. Both the surfaces and the cross-sections of the samples were investigated. Detailed studies were made using scanning electron microscopy (SEM), XRD, metallographic microscopy, chemical composition analysis and fractal analysis. The characteristics of the surface geometry involved such parameters as circularity, roundness, solidity, Feret\'s diameter, watershed diameter, fractal dimensions and corner frequencies, which were calculated by numerical processing of SEM images.
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  • 文章类型: Journal Article
    本研究通过蒙特卡罗模拟研究了样品固有温度对具有不同侧壁角的硅梯形线的线扫描轮廓的影响。这项研究表明,轮廓随温度而变化,特别关注“肩膀”,这变得更加明显与较大的侧壁角度。随着温度升高,二次电子分布的对比度在低初级电子能量下增加,但在相对高的PE能量下降低。反向散射电子分布的趋势相似,但不太明显。详细讨论了潜在的机制。这项研究有可能为使用SEM在纳米结构中进行测温提供有价值的见解。
    This study investigates the influence of the sample inherent temperature on the line-scan profile for a silicon trapezoid line with different sidewall angles by Monte-Carlo simulation. This study demonstrates that the profile varies with temperature, particularly focusing on the \'shoulder\', which becomes more pronounced with larger sidewall angles. The contrast of the secondary electron profile increases at low primary electron energy but decreases at relatively high PE energy as the temperature rises. The trend of the backscattering electron profile is similar but less noticeable. The underlying mechanism is discussed in detail. This study has potential to provide valuable insights into thermometry in nanostructures using SEMs.
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  • 文章类型: Journal Article
    使用扫描电子显微镜(SEM),对来自伊朗的六种Salsola物种的表皮进行了检查,种子,和果实微观形态。其中包括来自Heterotricha部分的腕虫,杜鹃花,S、incanescens,和来自Caroxylon部分的东方链球菌,卡利区的S.卡利,和来自Physurus科的Turcomanica。表皮细胞分为三种类型。有钻石,不规则,和多边形细胞,以及笔直和起伏的墙壁。研究过的Salsola物种具有光滑或雕刻的果实表面,水果表面装饰有三种主要类型。根据头发的类型和果实的密度,这些物种之间存在显着差异。种子形状和颜色没有什么系统意义。种子表皮由多边形组成,细长的多边形,不规则,钻石细胞虽然多边形和不规则的睾丸细胞是最常见的,它们的大小和形状可以在特异性和特异性水平上提供额外的信息和有用的诊断特征.对于分类分离,当前的研究提供了微形态学水平的新见解。研究重点:本文报道了盐生植物作为适应极端栖息地的模型。这些植物位于旱生植物的生态群落中。这里,第一次,已经研究了Salsola的微观结构分析。此外,它提供了对植物物种对极端条件的反应的新见解,以及微形态学水平上可能的适应策略。
    Using scanning electron microscopy (SEM), six Salsola species from Iran were examined for their epidermis, seed, and fruit micromorphology. Among them were S. brachiata from section Heterotricha, S dendroides, S. incanescens, and S. orientalis from section Caroxylon, S. kali from section Kali, and S. turcomanica from section Physurus. Epidermal cells are divided into three types. There were diamond, irregular, and polygonal cells, as well as straight and undulated walls. Studied species of Salsola have smooth or sculptured fruit surfaces, and there are three main types of fruit surface ornamentation. There is a significant difference between these species based on the type of hair and density of the fruit. Seed shape and color have little systematic significance. The seed epidermis is composed of polygonal, elongated polygonal, irregular, and diamond cells. Although polygonal and irregular testa cells are most common, their size and shape can provide additional information and useful diagnostic characteristics at both specific and infraspecific levels. For taxonomic separation, the current study provides novel insights at micromorphological levels. RESEARCH HIGHLIGHTS: This article reports halophyte are shown as models for adaptation to extreme habitats. These plants are placed among the ecological communities of xerophytes. Here, for the first time, the microstructural analysis of Salsola has been investigated. Additionally, it provides new insights into plant species\' response to extreme conditions, as well as possible adaptation strategies at the micromorphological level.
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