■癌症干细胞(CSC)在很大程度上影响结直肠癌(CRC)的发展,转移,复发,和对治疗的抵抗力。布洛芬和热疗可有效治疗癌症。在这里,我们评估了热疗和布洛芬对分离的CRC-CSC的影响。
■这项实验研究于2020年9月至2022年1月在病理学系进行,医学院,ShirazUniversityofMedicalSciences,伊朗。使用非粘附培养系统从HT-29细胞中分离CSC。为了确认分离的CSC的干性,通过实时定量PCR(qRT-PCR)和流式细胞术检测,评估了干性基因和蛋白质标志物的表达。用热疗和布洛芬处理分离的CSC。通过MTT测定和锥虫蓝染色确定细胞活力。stemness的表达,扩散,通过qRT-PCR评估Wnt信号通路和凋亡基因。
■CSC在14天内分离。CD-133标记和OCT3/4,C-MYC的表达,分离的CSC中KLF4和NANOG基因高于HT-29细胞(P<0.05)。处理的CSC的细胞活力显著降低(P<0.05)。热疗降低OCT3/4、NANOG、PCNA,WNT1和CTNNB1基因的表达与P53、BAX、和KLF4基因(P<0.05)。布洛芬降低OCT3/4、BCL2、NANOG、PCNA,WNT1和CTNNB1基因的表达与P53、BAX、和KLF4基因在处理的CSC中(P<0.05)。
■热疗和布洛芬治疗表现出对结肠直肠CSC的抑制作用。然而,使用联合疗法仍有待测试。
UNASSIGNED: Cancer stem cells (CSCs) substantially influence the development of colorectal cancer (CRC), metastasis, relapse, and resistance to therapy. Ibuprofen and hyperthermia can be effective in the treatment of cancer. Herein, we evaluated the effects of hyperthermia and ibuprofen on the isolated-CSCs of CRC.
UNASSIGNED: This experimental study was conducted between Sep 2020 and Jan 2022 at the Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Iran. A non-adhesive culture system was used to isolate CSCs from HT-29 cells. To confirm the stemness nature of isolated-CSCs, the expression of stemness genes and protein markers was evaluated by quantitative Real-time PCR (qRT-PCR) and flow cytometry assay. The isolated-CSCs were treated with hyperthermia and ibuprofen. The cell viability was determined by MTT assay and trypan blue staining. The expression of stemness, proliferation, Wnt signaling pathway and apoptosis genes was assessed by qRT-PCR.
UNASSIGNED: CSCs were isolated within 14 days. The expression of CD-133 marker and OCT3/4, C-MYC, KLF4, and NANOG genes in isolated-CSCs was higher than HT-29 cells (P<0.05). Cell viability of treated-CSCs were considerably reduced (P<0.05). Hperthermia reduced the expression of OCT3/4, NANOG, PCNA, WNT1 and CTNNB1 genes and increased the expression of P53, BAX, and KLF4 genes (P<0.05). Ibuprofen decreased the expression of OCT3/4, BCL2, NANOG, PCNA, WNT1, and CTNNB1 genes and increased the expression of P53, BAX, and KLF4 genes in treated-CSCs (P<0.05).
UNASSIGNED: Hyperthermia and ibuprofen treatment demonstrate an inhibitory effect on colorectal CSCs. However, using combination therapy is remaining to be tested.