Abstract：
Coregulation of microRNAs (miRNAs) and cancer stem cells (CSCs) is very important in carcinogenesis. miR-127-5p is known to be downregulated in breast cancer. In this study, we aimed to investigate how boric acid (BA), known for its previously unstudied anti-cancer properties, would affect the expression of miR127-5p and genes responsible for breast cancer stem cells (BC-SCs) metastasis. BC-SCs were isolated from human breast cancer cells (MCF-7) by immunomagnetic cell separation and characterized with flow cytometry and sphere formation. The viability of BC-SCs and the determination of its IC50 value in response to boric acid (BA) were assessed via the MTT assay. Boric acid exhibited dose- and time-dependent inhibition of cell viability in cells. The IC50 doses of boric acid in MCF-7 cells and BC-SCs were 45.69 mM and 41.27 mM, respectively. The impact of BA on the expression of metastatic genes and miR127-5p was elucidated through RT-qPCR analysis. While the expression of the COL1A1 (p < 0.05) and VIM (p < 0.01) was downregulated, the expression of the miR-127-5p, ZEB1 (p < 0.01), CDH1 (p < 0.05), ITGB1 (p < 0.05), ITGA5 (p < 0.05), LAMA5 (p < 0.01), and SNAIL (p < 0.05), was up-regulated in dose-treated BC-SCs (p < 0.001) to the RT-qPCR results. Our findings suggest that boric acid could induce miR-127-5p expression. However, it cannot be said that it improves the metastasis properties of breast cancer stem cells.
摘要：
microRNAs(miRNAs)和肿瘤干细胞(CSCs)的共调控在肿瘤发生过程中非常重要。已知miR-127-5p在乳腺癌中下调。在这项研究中,我们的目的是研究硼酸(BA)，以其先前未研究的抗癌特性而闻名，会影响miR127-5p的表达和负责乳腺癌干细胞（BC-SC）转移的基因。通过免疫磁性细胞分离从人乳腺癌细胞(MCF-7)中分离BC-SC,并用流式细胞术和球体形成表征。通过MTT测定评估BC-SC的活力及其响应于硼酸(BA)的IC50值的测定。硼酸对细胞中的细胞活力表现出剂量和时间依赖性的抑制。MCF-7细胞和BC-SCs中硼酸的IC50剂量分别为45.69mM和41.27mM,分别。通过RT-qPCR分析阐明了BA对转移基因和miR127-5p表达的影响。而COL1A1(p<0.05)和VIM(p<0.01)的表达下调,miR-127-5p的表达，ZEB1（p<0.01），CDH1（p<0.05），ITGB1（p<0.05），ITGA5（p<0.05），LAMA5（p<0.01），和蜗牛（p<0.05），与RT-qPCR结果相比,在剂量处理的BC-SC中上调(p<0.001)。我们的发现表明硼酸可以诱导miR-127-5p表达。然而,不能说它提高了乳腺癌干细胞的转移特性。
