Abstract：
A cancer mass is composed of a heterogeneous group of cells, a small part of which constitutes the cancer stem cells since they are less differentiated and have a high capacity to develop cancer. Versican is an extracellular matrix protein located in many human tissues. The mRNA of versican has been shown to have \"splicing patterns\" as detected by RT-PCR, northern blot analysis, and cDNA sequencing. Based on this knowledge this study aims to reveal the splice variants of versican molecules, which are thought to be involved in the pathogenesis of the DU-145 human prostatic carcinoma cell line and prostatic cancer stem cells isolated from this cell line. In this study, RWPE-1 normal prostatic and DU-145 human prostate cancer cell lines have been used. Prostatic cancer stem cells and the remaining group of non-prostatic-cancer stem cells (bulk population) were isolated according to their CD133+/CD44+. RNA was isolated in all groups, and sequence analysis was accomplished for splicing variants by Illumina NextSeq 500 sequencing system. The results were analyzed by bioinformatic evaluation. As five isoforms of the versican gene in the differential transcript expression are analyzed, it was observed that a significant change was only found in the isoforms Versican 0 and Versican 1. In this study, we explored the function of this molecule which we think to be effective in cancer progression, and suggested that more valuable results can be obtained after the accomplishment of in vivo experiments.
摘要：
癌团由一组异质细胞组成，其中一小部分构成癌症干细胞，因为它们分化程度较低，并且具有很高的发展癌症的能力。Versican是位于许多人体组织中的细胞外基质蛋白。通过RT-PCR检测，versican的mRNA已显示具有“剪接模式”，northern印迹分析,和cDNA测序。基于这些知识，本研究旨在揭示versican分子的剪接变体，它们被认为与DU-145人前列腺癌细胞系和从该细胞系分离的前列腺癌干细胞的发病机理有关。在这项研究中,已使用RWPE-1正常前列腺和DU-145人前列腺癌细胞系。根据它们的CD133+/CD44+分离前列腺癌干细胞和剩余的非前列腺癌干细胞组(大量群体)。在所有组中分离RNA，通过IlluminaNextSeq500测序系统完成剪接变体的序列分析。通过生物信息学评价对结果进行分析。由于分析了差异转录表达中versican基因的五种同工型，观察到仅在同种型Versican0和Versican1中发现了显着变化。在这项研究中,我们探索了这种分子的功能，我们认为这种分子在癌症进展中有效，并建议在完成体内实验后可以获得更有价值的结果。
