■本研究探索了丝瓜海绵(LS)在增强乙酸化过程中的利用。LS以具有高孔隙率和比表面积而闻名,并可以提供一种支持乙酸细菌(AAB)生长的新手段,以提高生物量产量和乙酸化率,从而促进更高效和可持续的食醋生产。此外,LS和涂有κ-角叉菜胶(LSK)的丝瓜海绵的有前途的潜力意味着它们可能代表着共同生产具有工业价值的生物产品的有效替代品,例如细菌纤维素(BC)和乙酸。
■LS和LSK在浸没式半连续乙酸化过程中用作巴氏醋杆菌UMCC2951的吸附剂。实验是在1Hz和32℃的温度下往复摇动下进行的。根据细胞干重(CDW)评估了两种系统(分别为LS-AAB和LSK-AAB)的性能,酸化率,和BC生物膜的形成。
■使用LS显著提高了醋化过程中的生物量产量,与浮游细胞获得的0.91mg/L相比,CDW为3.34mg/L。用κ-角叉菜胶涂覆LS进一步提高了产量,CDW为4.45mg/LLSK-AAB系统的固化率也较高,达到3.33±0.05g/Ld,而LS-AAB为2.45±0.05g/Ld,浮游细胞为1.13±0.05g/Ld。此外,在第二个操作周期中,BC生物膜的形成在LSK-AAB系统中更为明显(37.0±3.0mg/L,与LS-AAB中的25.0±2.0mg/L相反)。
■这项研究表明,LS显着提高了乙酸化过程的效率,特别是用κ-角叉菜胶增强时。生物量产量的增加,加速乙化,增强的BC生物膜形成突出了LS-AAB系统的潜力,尤其是LSK-AAB变体,在可持续和有效的食醋生产中。这些系统为小规模,符合生态友好做法并满足专门市场需求的半连续乙化工艺。最后,这种创新的方法促进了乙酸和细菌纤维素的双重生产,在生物技术领域具有潜在的应用。
UNASSIGNED: This study explored the utilization of luffa sponge (LS) in enhancing acetification processes. LS is known for having high porosity and specific surface area, and can provide a novel means of supporting the growth of acetic acid bacteria (AAB) to improve biomass yield and acetification rate, and thereby promote more efficient and sustainable vinegar production. Moreover, the promising potential of LS and luffa sponge coated with κ-carrageenan (LSK) means they may represent effective alternatives for the co-production of industrially valuable bioproducts, for example bacterial cellulose (BC) and acetic acid.
UNASSIGNED: LS and LSK were employed as adsorbents for Acetobacter pasteurianus UMCC 2951 in a submerged semi-continuous acetification process. Experiments were conducted under reciprocal shaking at 1 Hz and a temperature of 32 °C. The performance of the two systems (LS-AAB and LSK-AAB respectively) was evaluated based on cell dry weight (CDW), acetification rate, and BC biofilm formation.
UNASSIGNED: The use of LS significantly increased the biomass yield during acetification, achieving a CDW of 3.34 mg/L versus the 0.91 mg/L obtained with planktonic cells. Coating LS with κ-carrageenan further enhanced yield, with a CDW of 4.45 mg/L. Acetification rates were also higher in the LSK-AAB system, reaching 3.33 ± 0.05 g/L d as opposed to 2.45 ± 0.05 g/L d for LS-AAB and 1.13 ± 0.05 g/L d for planktonic cells. Additionally, BC biofilm formation during the second operational cycle was more pronounced in the LSK-AAB system (37.0 ± 3.0 mg/L, as opposed to 25.0 ± 2.0 mg/L in LS-AAB).
UNASSIGNED: This study demonstrates that LS significantly improves the efficiency of the acetification process, particularly when enhanced with κ-carrageenan. The increased biomass yield, accelerated acetification, and enhanced BC biofilm formation highlight the potential of the LS-AAB system, and especially the LSK-AAB variant, in sustainable and effective vinegar production. These systems offer a promising approach for small-scale, semi-continuous acetification processes that aligns with eco-friendly practices and caters to specialized market needs. Finally, this innovative method facilitates the dual production of acetic acid and bacterial cellulose, with potential applications in biotechnological fields.