Abstract：
In this paper, the work has been done to develop a cost-effective methodology, for the isolation of the potential producer of bacterial nanocellulose. No report is available in the literature, on the use of gram flour and table sugar for the screening of nanocellulose-producing isolates. Since commercially used, Hestrin-Schramm medium is expensive for the isolation of nanocellulose-producing micro-organisms, the possibility of using gram flour-table sugar medium was investigated in this work. Qualitative screening of micro-organisms was done using cost-effective medium, i.e., gram flour-table sugar medium. Qualitative analysis of various nanocellulose-producing bacteria depicted that cellulose layer production occurred on both HS medium and gram flour-table sugar medium. The yield of nanocellulose was also better on air-liquid surface in case of gram flour-table sugar medium as compared to HS medium. 16S rRNA was used for molecular characterization of bacterial strain and the best nanocellulose producer was identified as Novacetimonas hansenii BMK-3_NC240423 (isolated from rotten banana). FTIR and FE-SEM studies of nanocellulose pellicle produced on HS medium and gram flour-table sugar medium demonstrated equivalent structural, morphological, and chemical properties. The cost of newly designed medium (0.01967 $/L) is nearly 90 times lower than the Hestrin-Schramm medium (1.748 $/L), which makes the screening of nanocellulose producers very cost-effective. A strategy of using gram flour extract-table sugar medium for the screening of nanocellulose-producing micro-organisms is a novel approach, which will drastically reduce the screening associated cost of cellulose-producing micro-organisms and also motivate the researchers/industries for comprehensive screening programme for getting high cellulose-producing microbes.
摘要：
在本文中,已经完成了开发具有成本效益的方法的工作，用于分离细菌纳米纤维素的潜在生产者。文献中没有报告，关于使用克面粉和食糖筛选产生纳米纤维素的分离株。自从商业使用以来，Hestrin-Schramm培养基对于分离产生纳米纤维素的微生物来说是昂贵的，在这项工作中研究了使用克面粉食糖培养基的可能性。使用具有成本效益的培养基对微生物进行定性筛选，即，克面粉-食糖培养基。对各种产生纳米纤维素的细菌的定性分析表明，在HS培养基和克面粉食糖培养基上都产生了纤维素层。与HS介质相比，在克面粉食糖介质的情况下，纳米纤维素的产量在空气-液体表面上也更好。16SrRNA用于细菌菌株的分子表征，最佳的纳米纤维素生产者被鉴定为NovacetimonashanseniiBMK-3_NC240423（从腐烂的香蕉中分离）。在HS培养基和克面粉制糖培养基上生产的纳米纤维素薄膜的FTIR和FE-SEM研究表明，形态学,和化学性质。新设计的介质（0.01967$/L）的成本比Hestrin-Schramm介质（1.748$/L）低近90倍，这使得筛选纳米纤维素生产商非常具有成本效益。使用克面粉提取物表糖培养基筛选产生纳米纤维素的微生物的策略是一种新颖的方法，这将大大降低生产纤维素的微生物的筛选相关成本，并激励研究人员/行业进行全面的筛选计划，以获得高生产纤维素的微生物。
