菌株I65T(=KACC22647T=JCM35315T),一个新的革兰氏染色阴性,严格的有氧,不活动,非孢子形成,杆状,用几种抗生素处理后,从废水处理系统的进水中分离出橙色细菌,比如美罗培南,庆大霉素,和大环内酯。新鉴定的细菌菌株I65T表现出显著的多药和重金属抗性特征。菌株I65T在Reasoner的2A培养基[0%-2%(w/v)NaCl(最佳,0%),pH5.0-10.0(最佳,pH7.0),和20-45°C(最佳,30°C)]。基于16SrRNA基因测序的系统发育分析证实,菌株I65T与燕山NiabellaCCBAU05354T密切相关(序列相似性为99.56%),希必西索利尼亚菌THG-DN5.5T(97.51%),和ginsengisoliNiabellaGR10-1T(97.09%)。对全基因组序列的进一步分析证实,数字DNA-DNA杂交,平均核苷酸同一性,菌株I65T和燕山N.CCBAU05354T之间的平均氨基酸同一性值分别为23.4、80.7和85.0%,分别,表明菌株I65T与燕山奈瑟菌不同。菌株I65T的基因组大小为6.1Mbp,根据牛津纳米孔平台的评估,其基因组DNAG+C含量为43.0mol%。菌株I65T的主要脂肪酸为iso-C15:0和iso-C15:1G,主要的呼吸醌是MK-7。此外,菌株I65T的主要极性脂质是磷脂酰乙醇胺。基于基因型,化学分类学,和表型数据,菌株I65T代表属于Niabella属的新物种,名字Niabelladefluviisp.11月。是提议的。菌株类型为I65T(=KACC22647T=JCM35315T)。
Strain I65T (=KACC 22647T=JCM 35315T), a novel Gram-stain-negative, strictly aerobic, non-motile, non-spore-forming, rod-shaped, and orange-pigmented bacterium was isolated from influent water of a wastewater treatment system after treatment with several antibiotics, such as meropenem, gentamicin, and macrolide. The newly identified bacterial strain I65T exhibits significant multi-drug and heavy metal resistance characteristics. Strain I65T was grown in Reasoner\'s 2A medium [0 %-2 % (w/v) NaCl (optimum, 0 %), pH 5.0-10.0 (optimum, pH 7.0), and 20-45°C (optimum, 30 °C)]. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain I65T was closely related to Niabella yanshanensis CCBAU 05354T (99.56 % sequence similarity), Niabella hibiscisoli THG-DN5.5T (97.51 %), and Niabella ginsengisoli GR10-1T (97.09 %). Further analysis of the whole-genome sequence confirmed that the digital DNA-DNA hybridization, average nucleotide identity, and average amino acid identity values between strain I65T and N. yanshanensis CCBAU 05354T were 23.4, 80.7, and 85.0 %, respectively, suggesting that strain I65T is distinct from N. yanshanensis. The genome size of strain I65T was 6.1 Mbp, as assessed using the Oxford Nanopore platform, and its genomic DNA G+C content was 43.0 mol%. The major fatty acids of strain I65T were iso-C15 : 0 and iso-C15 : 1 G, and the major respiratory quinone was MK-7. Moreover, the major polar lipid of strain I65T was phosphatidylethanolamine. Based on genotypic, chemotaxonomic, and phenotype data, strain I65T represents a novel species belonging to the genus Niabella, for which the name Niabella defluvii sp. nov. is proposed. The type strain is I65T (=KACC 22647T=JCM 35315T).