Abstract：
A Gram-stain-positive, rod-shaped bacterium, designated as HLT2-17T, was isolated from soil sample taken from the Hailuogou glacier in Sichuan province, PR China. Strain HLT2-17T was capable of growing at 4-25°C and in NaCl concentrations ranging from 0 to 2% (w/v). The highest level of 16S rRNA gene sequence similarity was observed with Pengzhenrongella phosphoraccumulans M0-14T (98.3 %) and Pengzhenrongella sicca LRZ-2T (98.2 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain HLT2-17T and its closest relatives, P. phosphoraccumulans M0-14T and P. sicca LRZ-2T, were 80.0-84.0 % and 23.3-27.7 %, respectively. Phylogenomic analysis indicated that strain HLT2-17T clustered together with strains P. phosphoraccumulans M0-14T and P. sicca LRZ-2T. Strain HLT2-17T contained C16 : 0 and anteiso-C15 : 0 as the major fatty acids, and MK-9(H4) as the menaquinone. Therefore, based on a polyphasic approach, we propose that strain HLT2-17T (=CGMCC 1.11116T= NBRC 110443T) represents a novel species of the genus Pengzhenrongella and suggest the name Pengzhenrongella frigida sp. nov.
摘要：
革兰氏染色阳性，杆状细菌,指定为HLT2-17T，从四川海螺沟冰川的土壤样本中分离出来，公关中国。菌株HLT2-17T能够在4-25°C和在0至2％（w/v）的NaCl浓度范围内生长。16SrRNA基因序列相似性水平最高的是彭振荣磷菌M0-14T(98.3%)和彭振荣磷菌LRZ-2T(98.2%)。HLT2-17T菌株及其近亲之间的平均核苷酸同一性和数字DNA-DNA杂交值，P.磷积累M0-14T和P.siccaLRZ-2T，分别为80.0-84.0%和23.3-27.7%，分别。系统基因组分析表明，菌株HLT2-17T与菌株P.phosphoracracansM0-14T和P.siccaLRZ-2T聚集在一起。菌株HLT2-17T含有C16:0和anteiso-C15:0作为主要脂肪酸，和MK-9（H4）作为甲基萘醌。因此,基于多相方法，我们认为菌株HLT2-17T（=CGMCC1.11116T=NBRC110443T）代表了彭珍怪菌属的一种新物种，并建议名称为Pengzhenrongellafrigidasp。11月。
