Abstract：
A Gram-stain-positive, rod-shaped, aerobic, motile bacterium, J379T, was isolated from radioactive water spring C1, located in a former silver-uranium mine in the Czech Republic. This slow-growing strain exhibited optimal growth at 24-28 °C on solid media with <1 % salt concentration and alkaline pH 8-10. The only respiratory quinone found in strain J379T was MK-7(H4). C18 : 1 ω9c (60.9 %), C18 : 0 (9.4 %), C16 : 0 and alcohol-C18 : 0 (both 6.2 %) were found to be the major fatty acids. The peptidoglycan contained directly cross-linked meso-diaminopimelic acid. Phylogenetic reconstruction based on the 16S rRNA gene sequences and the core-genome analysis revealed that strain J379T forms a separate phylogenetic lineage within the recently amended order Solirubrobacterales. A comparison of the 16S rRNA gene sequences between strain J379T and other members of the order Solirubrobacterales showed <96 % similarity. This analysis revealed that the closest type strains were Parviterribacter kavangonensis D16/0 /H6T (95.2 %), Capillimicrobium parvum 0166_1T (94.9 %) and Conexibacter arvalis KV-962T (94.5 %). Whole-genome analysis showed that the closest type strain was Baekduia soli BR7-21T with an average nucleotide identity of 78 %, average amino acid identity of 63.2 % and percentage of conserved proteins of 48.2 %. The G+C content of the J379T genomic DNA was 71.7 mol%. Based on the phylogenetic and phylogenomic data, as well as its physiological characteristics, strain J379T is proposed to represent a type strain (DSM 113746T=CCM 9300T) of Svornostia abyssi gen. nov. sp. nov. within the family Baekduiaceae.
摘要：
革兰氏染色阳性，杆状,有氧,活动细菌，J379T,是从位于捷克共和国以前的银铀矿中的放射性水泉C1中分离出来的。这种缓慢生长的菌株在24-28°C下在〈1%盐浓度和碱性pH8-10的固体培养基上表现出最佳生长。在菌株J379T中发现的唯一呼吸醌是MK-7(H4)。C18：1ω9c（60.9%），C18：0（9.4%），发现C16:0和醇-C18:0(均为6.2%)是主要的脂肪酸。肽聚糖含有直接交联的内消旋二氨基庚二酸。基于16SrRNA基因序列和核心基因组分析的系统发育重建表明，菌株J379T在最近修订的Solirubrobacterales顺序中形成了单独的系统发育谱系。菌株J379T与Solirubrobactericales的其他成员之间的16SrRNA基因序列的比较显示<96％的相似性。该分析表明，最接近的菌株是卡文细小杆菌D16/0/H6T（95.2％），细小细菌0166_1T(94.9%)和阿维氏杆菌属KV-962T(94.5%)。全基因组分析表明，最接近的菌株是BaekduiasoliBR7-21T，平均核苷酸同一性为78％，平均氨基酸同一性为63.2%，保守蛋白百分比为48.2%。J379T基因组DNA的G+C含量为71.7mol%。根据系统发育和系统发育数据，以及它的生理特征，菌株J379T被提议代表一种深渊丝裂菌的类型菌株(DSM113746T=CCM9300T)。11月。sp.11月。在百度科。
