Abstract：
A Gram-stain-positive, aerobic bacterium, designated as YPD9-1T, was isolated from the gut contents of a spotty belly greenling, Hexagrammos agrammus, collected near Dokdo island, South Korea. The rod-shaped cells were oxidase-positive, and catalase-negative. The major cellular fatty acids were anteiso-C15 : 0, iso-C15 : 0, C16 : 0, iso-C16 : 0 and iso-C17: 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 47.6 mol% and the predominant respiratory quinone was menaquinone MK-7. The 16S rRNA gene sequence of YPD9-1T showed low sequence similarities to species of the genus Paenibacillus, Paenibacillus pocheonensis Gsoil 1138T (97.21 % of sequence similarity), Paenibacillus aestuarii CJ25T (97.12 %) and Paenibacillus allorhizoplanae JJ-42T (96.89 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that YPD9-1T formed a distinct branch among other species of the genus Paenibacillus. The digital DNA-DNA hybridisation, average nucleotide identity, and average amino acid identity values between YPD9-1T and the related species were in the ranges of 15.3-16.2 %, 74.1-78.4 %, and 71.1-71.9 %, respectively, which are below the species cutoff values. On the basis of the results of the polyphasic analysis, we conclude that strain YPD9-1T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus hexagrammi sp. nov. is proposed. The type strain of Paenibacillus hexagrammi is YPD9-1T (=KCTC 43424T =LMG 32988T).
摘要：
革兰氏染色阳性，需氧细菌，指定为YPD9-1T，从腹部斑点绿色的肠道内容物中分离出来，Hexagrammosagrammus,收集在独岛附近,韩国。杆状细胞为氧化酶阳性,过氧化氢酶阴性.主要的细胞脂肪酸是antiso-C15:0、iso-C15:0、C16:0、iso-C16:0和iso-C17:0。主要的极性脂质是二磷脂酰甘油，磷脂酰甘油，磷脂酰乙醇胺和两种未鉴定的脂质。DNAGC含量为47.6mol％，主要的呼吸醌是甲萘醌MK-7。YPD9-1T的16SrRNA基因序列显示出与类芽孢杆菌属的低序列相似性，石鸡类芽孢杆菌1138T(序列相似性97.21%),阿氏芽孢杆菌CJ25T(97.12%)和阿氏芽孢杆菌JJ-42T(96.89%)。基于16SrRNA基因序列的系统发育分析结果表明，YPD9-1T在类芽孢杆菌属的其他物种中形成了独特的分支。数字DNA-DNA杂交,平均核苷酸同一性，YPD9-1T与相关物种的平均氨基酸同一性值在15.3-16.2％之间，74.1-78.4%，和71.1-71.9％，分别,低于物种截止值。根据多相分析的结果，我们得出的结论是，菌株YPD9-1T代表了类芽孢杆菌属的一种新物种，它的名字叫六面芽孢杆菌。11月。是提议的。六方芽孢杆菌的类型菌株是YPD9-1T(=KCTC43424T=LMG32988T)。
