关键词: 16S rRNA gene aequorivita draft genome sequencing novel species

Mesh : RNA, Ribosomal, 16S / genetics Geologic Sediments / microbiology Phylogeny Fatty Acids / chemistry China Base Composition Vitamin K 2 / analogs & derivatives analysis Bacterial Typing Techniques DNA, Bacterial / genetics Sequence Analysis, DNA Seawater / microbiology Molecular Sequence Data Phospholipids / chemistry Phosphatidylethanolamines

来  源:   DOI:10.1099/ijsem.0.006423

Abstract:
Two Gram-stain-negative, rod-shaped, non-motile, strictly aerobic strains, forming yellow colonies and designated F6058T and S2608T, were isolated from marine sediment collected in Weihai, PR China. Both strains grow at 4-40 °C (optimum, 30-33 °C), pH 6.0-7.5 (optimum, pH 6.5) and in the presence of 0-7.0 % (w/v) NaCl. The optimum NaCl concentrations for strains F6058T and S2608T were 2.0 % and 2.5 %, respectively. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strains F6058T and S2608T share an evolutionary lineage with members of the genus Aequorivita. The isolates exhibited a 16S rRNA gene sequence similarity of 96.7 % to each other. Strains F6058T exhibited the highest 16S rRNA gene sequence similarity to Aequorivita xiaoshiensis F64183T (98.8 %), and S2608T was most similar to Aequorivita capsosiphonis A71T (96.9 %). Iso-C15:0, anteiso-C15:0 and iso-C17:0 3-OH were the major fatty acids of strains F6058T and S2608T. The sole respiratory quinone of both isolates was menaquinone 6 (MK-6). The polar lipid profiles of the isolates both consisted of phosphatidylethanolamine and phosphoglycolipids; however, strain F6058T exhibited one glycolipid, one aminolipid and two unidentified polar lipids, and strain S2608T also had two glycolipids and one unidentified polar lipid. The DNA G+C contents of strains F6058T and S2608T were 34.6 % and 37.7 mol%, respectively. Based on their phenotypic, chemotaxonomic and genomic characteristics, strains F6058T and S2608T were considered to represent novel species of the genus Aequorivita, for which the names Aequorivita sediminis sp. nov. and Aequorivita marina sp. nov. were proposed. The type strains are F6058T (=KCTC 92653T=MCCC 1H01358T) and S2608T (KCTC 92652T=MCCC 1H01361T).
摘要:
两个革兰氏染色阴性,杆状,不活动,严格的有氧菌株,形成黄色菌落,命名为F6058T和S2608T,从威海收集的海洋沉积物中分离出来,公关中国。两种菌株均在4-40°C下生长(最佳,30-33°C),pH6.0-7.5(最佳,pH6.5)和在0-7.0%(w/v)NaCl存在下。菌株F6058T和S2608T的最佳NaCl浓度分别为2.0%和2.5%,分别。16SrRNA基因序列的系统发育分析表明,菌株F6058T和S2608T与Aequorivita属的成员共享进化谱系。分离株的16SrRNA基因序列相似性为96.7%。菌株F6058T与小石子F64183T的16SrRNA基因序列相似性最高(98.8%),S2608T与水牛A71T最相似(96.9%)。Iso-C15:0,anteiso-C15:0和iso-C17:03-OH是菌株F6058T和S2608T的主要脂肪酸。两种分离物的唯一呼吸醌是甲基萘醌6(MK-6)。分离物的极性脂质分布均由磷脂酰乙醇胺和磷酸糖脂组成;然而,菌株F6058T表现出一种糖脂,一种氨基脂质和两种未知的极性脂质,菌株S2608T还具有两种糖脂和一种未鉴定的极性脂质。菌株F6058T和S2608T的DNAGC含量分别为34.6%和37.7mol%,分别。根据他们的表型,化学分类学和基因组特征,菌株F6058T和S2608T被认为代表了Aequorivita属的新物种,名称为Aequorivitasediminissp。11月。和Aequorivitamarinasp.11月。被提议。菌株类型为F6058T(=KCTC92653T=MCCC1H01358T)和S2608T(KCTC92652T=MCCC1H01361T)。
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