Abstract：
Two myxobacterial strains (KH5-1T and NO1) were isolated from the activated sludge tanks treating municipal sewage wastewater in Japan. These strains were recognised as myxobacteria based on their phenotypic characteristics of swarming colonies and fruiting bodies. Phylogenetic analyses using the 16S rRNA gene revealed that strains KH5-1T and NO1 were affiliated with the genus Corallococcus, with the closest neighbours being Corallococcus exercitus AB043AT (99.77% and 99.84%, respectively). Genome comparisons using orthologous average nucleotide identity (orthoANI) and digital DNA-DNA hybridisation similarity (dDDH) with strains KH5-1T and NO1 and their phylogenetically close relatives in Corallococcus spp. were below the thresholds. The major cellular fatty acids of strains KH5-1T and NO1 were iso-C15:0 (31.9%, 30.0%), summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) (20.2%, 17.7%), and iso-C17:0 (12.1%, 14.8%), and the major respiratory quinone was found to be menaquinone (MK)-8. Based on the phenotypic, chemotaxonomic, and phylogenetic evidence, strains KH5-1T and NO1 represent a new species in the genus Corallococcus, for which the proposed name is Corallococcus caeni sp. nov. The type strain is KH5-1T (= NCIMB 15510T = JCM 36609T).
摘要：
从日本处理城市污水的活性污泥池中分离出两种粘细菌菌株（KH5-1T和NO1）。根据蜂群菌落和子实体的表型特征，这些菌株被认为是粘细菌。使用16SrRNA基因进行的系统发育分析显示，菌株KH5-1T和NO1与头球菌属相关，最近的邻居是红球菌AB043AT（99.77％和99.84％，分别)。使用直系同源平均核苷酸同一性（orthoANI）和数字DNA-DNA杂交相似性（dDDH）与菌株KH5-1T和NO1及其在Corallococusspp中的系统发育近亲进行基因组比较。低于门槛。菌株KH5-1T和NO1的主要细胞脂肪酸为iso-C15:0(31.9%,30.0%），求和特征3(包括C16:1ω7c和/或C16:1ω6c)(20.2%，17.7%），和iso-C17：0(12.1%，14.8%），发现主要的呼吸醌是甲基萘醌（MK）-8。根据表型，化学分类学，和系统发育证据，菌株KH5-1T和NO1代表了球菌属的新物种，建议的名称是caeni珊瑚。11月。菌株类型为KH5-1T(=NCIMB15510T=JCM36609T)。
