■口腔微生物群暴露的牙髓对三重抗生素糊剂(TAP)的反应,环丙沙星的混合物,甲硝唑,和米诺环素与聚乙二醇和丙二醇软膏,仍需在细胞水平上完全澄清。这项研究旨在阐明暴露于口腔微生物群的牙髓对小鼠磨牙中TAP覆盖的反应。
■在6周龄小鼠的第一磨牙上准备一个洞,暴露牙髓24小时。暴露的牙髓用TAP(TAP组)或氢氧化钙水泥(CH组)盖住,除了聚乙二醇(M)和丙二醇(P)(MP,对照组),然后是玻璃离聚物水泥填充。以1、2和3周的间隔收集样品,除了定量实时聚合酶链反应(qRT-PCR)分析外,还进行了巢蛋白和Ki-67的免疫组织化学和脱氧尿苷-5'-三磷酸生物素缺口末端标记(TUNEL)测定。
■在第2周和第3周,对照组的牙髓坏死发生率最高,其次是CH组,而观察到牙髓愈合区域的发生率最高。TAP组在每个时间点。在第2周,在TAP组的牙髓中首先观察到三级牙本质形成。相比之下,在CH组中经常观察到骨样和/或纤维组织.qRT-PCR分析阐明,TAP分别在第1周和第2周激活干细胞和树突细胞。
■使用TAP作为牙髓覆盖剂改善了小鼠磨牙中口腔-微生物区系暴露的牙髓的愈合过程。
UNASSIGNED: Responses of oral-microflora-exposed dental pulp to a triple antibiotic paste (TAP), a mixture of ciprofloxacin, metronidazole, and minocycline in ointment with macrogol and propylene glycol, remain to be fully clarified at the cellular level. This study aimed to elucidate responses of oral-microflora-exposed dental pulp to capping with TAP in mouse molars.
UNASSIGNED: A cavity was prepared on the first molars of 6-week-old mice to expose the dental pulp for 24 h. The exposed pulp was capped with TAP (TAP group) or calcium hydroxide cement (CH group), in addition to the combination of macrogol (M) and propylene glycol (P) (MP, control group), followed by a glass ionomer cement filling. The samples were collected at intervals of 1, 2, and 3 weeks, and immunohistochemistry for nestin and Ki-67 and deoxyuride-5\'-triphosphate biotin nick end labeling (TUNEL) assay were performed in addition to quantitative real-time polymerase chain reaction (qRT-PCR) analyses.
UNASSIGNED: The highest occurrence rate of pulp necrosis was found in the control group followed by the CH group at Weeks 2 and 3, whereas the highest occurrence rate of healed areas in the dental pulp was observed in the TAP group at each time point. Tertiary dentin formation was first observed in the dental pulp of the TAP group at Week 2. In contrast, bone-like and/or fibrous tissues were frequently observed in the CH group. qRT-PCR analyses clarified that TAP activated the stem and dendritic cells at Weeks 1 and 2, respectively.
UNASSIGNED: The use of TAP as a pulp-capping agent improved the healing process of oral-microflora-exposed dental pulp in mouse molars.