背景:犬细小病毒2型(CPV-2)是感染犬的最常见的肠道病毒。CPV是主要由狗的临床胃肠道体征定义的传染病的病原体。在1970年代后期,CPV-2是一种能够感染家犬并在世界各地生长的新病毒。VP2基因是致病性的关键决定因素,抗原性,和CPV-2的宿主相互作用。
目的:VP2基因的分子特征对于了解CPV进化和流行病学至关重要。
方法:对编码VP2蛋白的基因进行测序并与全世界的参考菌株进行比较。最大似然法用于使用CPVVP2基因核苷酸序列构建系统发育树。
结果:我们对VP2基因的系统发育分析显示,5个菌株非常相似,并且聚集在一起,三个菌株在2b进化枝中,而其他两个在2a/2b进化枝。
结论:本文报道了两种新型CPV-2a/2b亚型在有胃肠道症状的犬中的分子特征。在包含编码结构蛋白VP2的开放阅读框(ORF)之一的CPV基因组区域上进行遗传分析。序列分析表明新的和未报告的序列变化,主要影响VP2基因,其中包括突变Ser297Ala和Leu87Met。这项研究代表了Türkiye中新的CPV-2a/2b亚型的第一个证据。由于VP2在编码CPV-2的衣壳蛋白中的关键作用及其在宿主-病毒相互作用中的重要参与,密切监测其进化变化并在寻找新的或预先存在的亚型时保持谨慎至关重要.
结论:这项研究强调了连续分子研究对于获得有关新型CPV突变体循环的更多见解的重要性。
BACKGROUND: Canine parvovirus type 2 (CPV-2) is the most common enteric virus that infects canids. CPV is the causative agent of a contagious disease defined mostly by clinical gastrointestinal signs in dogs. During the late 1970s, CPV-2 emerged as a new virus capable of infecting domestic dogs and growing across the world. The VP2 gene stands out as a key determinant in the pathogenicity, antigenicity, and host interactions of CPV-2.
OBJECTIVE: The molecular characterization of the VP2 gene is crucial for understanding CPV evolution and epidemiology.
METHODS: Genes encoding the VP2 protein were sequenced and compared to reference strains worldwide. The maximum likelihood method was used to build a phylogenetic tree using CPV VP2 gene nucleotide sequences.
RESULTS: Our phylogenetic analysis of the VP2 gene revealed that five strains were very similar and clustered together, and three strains were in the 2b clade, whereas the other two were in the 2a/2b clade.
CONCLUSIONS: This paper reports the molecular characterization of two novel CPV-2a/2b subtypes in dogs with gastrointestinal symptoms. Genetic analysis was conducted on a CPV genomic region encompassing one of the open reading frames (ORFs) encoding the structural protein VP2. Sequence analysis indicates new and unreported sequence changes, mainly affecting the VP2 gene, which includes the mutations Ser297Ala and Leu87Met. This study represents the first evidence of a new CPV-2a/2b subtype in Türkiye. Due to VP2\'s crucial role in encoding the capsid protein of CPV-2 and its significant involvement in the host-virus interaction, it is critical to closely monitor its evolutionary changes and be cautious while searching for novel or pre-existing subtypes.
CONCLUSIONS: This study highlights the significance of continuous molecular research for acquiring more insights on the circulation of novel CPV mutants.