Abstract：
The diversity and evolution of the genomes of human bocavirus (HBoV), which causes respiratory diseases, have been scarcely studied. Here, we aimed to obtain and characterize HBoV genomes from patients\'s nasopharyngeal samples collected between 2017 and 2022 period (5 years and 7 months). Next-generation sequencing (NGS) used Illumina technology after having implemented using GEMI an in-house multiplex PCR amplification strategy. Genomes were assembled and analyzed with CLC Genomics, Mafft, BioEdit, MeV, Nextclade, MEGA, and iTol. A total of 213 genomes were obtained. Phylogeny classified them all as of Bocavirus 1 (HBoV1) species. Five HBoV1 genotypic clusters determined by hierarchical clustering analysis of 27 variable genome positions were scattered over the study period although with differences in yearly prevalence. A total of 167 amino acid substitutions were detected. Besides, coinfection was observed for 52% of the samples, rhinoviruses then adenoviruses (HAdVs) being the most common viruses. Principal component analysis showed that HBoV1 genotypic cluster α tended to be correlated with HAdV co-infection. Subsequent HAdV typing for HBoV1-positive samples and negative controls demonstrated that HAdVC species predominated but HAdVB was that significantly HBoV1-associated. Overall, we described here the first HBoV1 genomes sequenced for France. HBoV1 and HAdVB association deserves further investigation.
摘要：
人类博卡病毒（HBoV）基因组的多样性和进化，导致呼吸道疾病，几乎没有被研究过。这里,我们旨在从2017年至2022年期间(5年7个月)收集的患者鼻咽样本中获取并表征HBoV基因组.下一代测序（NGS）在使用GEMI实施了内部多重PCR扩增策略后，使用了Illumina技术。用CLC基因组学组装和分析基因组，Mafft,BioEdit,MeV,Nextclade,MEGA,还有iTol.共获得213个基因组。系统发育将它们全部分类为Bocavirus1（HBoV1）物种。在研究期间，通过对27个可变基因组位置的层次聚类分析确定的5个HBoV1基因型簇分散在研究期间，尽管年患病率存在差异。检测到总共167个氨基酸取代。此外,在52%的样本中观察到共感染，鼻病毒，然后腺病毒（HAdV）是最常见的病毒。主成分分析表明，HBoV1基因型簇α与HAdV共感染有一定的相关性。随后对HBoV1阳性样品和阴性对照的HAdV分型表明，HAdVC物种占主导地位，但HAdVB与HBoV1显着相关。总的来说,我们在这里描述了为法国测序的第一个HBoV1基因组。HBoV1与HAdVB的相关性值得进一步研究。
