Amdoparvov感染各种食肉动物,包括芥菜,犬科动物,臭鼬,还有Felids.阿留申水貂病病毒(AMDV)属于典型的食肉动物氨虫病毒1。这里,我们在养殖的亚洲badge(Melesmeles)中发现了一种新型的氨纶病毒,我们将这种病毒命名为“Melesmelesamdoparvovirus”(MMADV)。共从134只个体badge中收集了146份临床样本,和30.6%(41/134)的采样of通过PCR检测出的氨甲病毒呈阳性。在粪便中检测到病毒DNA,血,脾,脾肝脏,肺,和这些动物的脂肪组织。测定了8个样本的病毒序列,其中五个代表几乎全长的基因组序列(4,237-4,265nt)。六份血清样本经PCR检测呈阳性,CIEP,和IAT,其中四个具有高的抗AMDV-G的抗体滴度(>512)。41只氨甲病毒阳性的badge中有26只显示出疾病迹象,尸检显示他们的器官有病变.这些badge的病毒NS1和VP2基因的序列比较和系统发育分析表明,它们的NS1蛋白与已知的氨虫病毒具有62.6%-88.8%的序列同一性,它们在系统发育上聚集成两个相关的进化枝。VP2蛋白共有76.6%-97.2%的同一性,并聚集成两个进化枝,其中之一包括浣熊狗和北极狐氨甲病毒(RFAV),另一种不包括其他已知的细菌病毒。根据基于NS1蛋白的细小病毒物种划分标准,来自YS农场的MMADV分离物应被归类为一种新的Amdoparvovovirus属物种的成员。总之,我们发现了一种新的MMADV和其他badge的氨苄病毒,它们自然感染亚洲badge,并且可能在badge中致病。
Amdoparvoviruses infect various carnivores, including mustelids, canids, skunks, and felids. Aleutian mink disease virus (AMDV) belongs to the prototypical species Amdoparvovirus carnivoran1. Here, we identified a novel amdoparvovirus in farmed Asian badgers (Meles meles), and we named this virus \"Meles meles amdoparvovirus\" (MMADV). A total of 146 clinical samples were collected from 134 individual badgers, and 30.6% (41/134) of the sampled badgers tested positive for amdoparvovirus by PCR. Viral DNA was detected in feces, blood, spleen, liver, lung, and adipose tissue from these animals. Viral sequences from eight samples were determined, five of which represented nearly full-length genome sequences (4,237-4,265 nt). Six serum samples tested positive by PCR, CIEP, and IAT, four of which had high antibody titers (> 512) against AMDV-G. Twenty-six of the 41 amdoparvovirus-positive badgers showed signs of illness, and necropsy revealed lesions in their organs. Sequence comparisons and phylogenetic analysis of the viral NS1 and VP2 genes of these badger amdoparvoviruses showed that their NS1 proteins shared 62.6%-88.8% sequence identity with known amdoparvoviruses, and they clustered phylogenetically into two related clades. The VP2 proteins shared 76.6%-97.2% identity and clustered into two clades, one of which included raccoon dog and arctic fox amdoparvovirus (RFAV), and the other of which did not include other known amdoparvoviruses. According to the NS1-protein-based criterion for parvovirus species demarcation, the MMADV isolate from farm YS should be classified as a member of a new species of the genus Amdoparvovirus. In summary, we have discovered a novel MMADV and other badger amdoparvoviruses that naturally infect Asian badgers and are possibly pathogenic in badgers.