尽管现代食品卫生取得了进步,由于微生物污染引起的食物中毒仍然是一个全球性问题,对人类健康构成了极大的威胁。尤其是,单核细胞增生李斯特菌和金黄色葡萄球菌是在具有生物膜的食物接触表面上发现的革兰氏阳性细菌。这些食源性病原体每年引起相当数量的食物中毒和感染。Ovomucin(OM)是蛋白中的水不溶性凝胶型糖蛋白。酶水解可用于提高OM的生物活性。本研究旨在调查是否使用五种商业酶(Alcalase®,菠萝蛋白酶,α-胰凝乳蛋白酶,木瓜蛋白酶,和胰酶)可以抑制单核细胞增生李斯特菌ATCC15313,单核细胞增生李斯特菌H7962,金黄色葡萄球菌KCCM11593和金黄色葡萄球菌7的生物膜形成。特别是,用木瓜蛋白酶(OMPP;500μg/mL)制备的OMH显着抑制了单核细胞增生李斯特菌ATCC15313,单核细胞增生李斯特菌H7962,金黄色葡萄球菌KCCM11593和金黄色葡萄球菌7的生物膜形成,80.28%,91.70%,79.00%,分别。此外,OMPP降低了代谢活性,胞外多糖生产(EPS),粘附能力,以及与这些细菌菌株的生物膜形成相关的基因表达。这些结果表明,OMH,尤其是OMPP,对单核细胞增生李斯特菌和金黄色葡萄球菌发挥抗生物膜作用。因此,OMPP可作为天然抗生物膜剂用于控制食品工业中的食物中毒。
Despite modern advances in food hygiene, food poisoning due to microbial contamination remains a global problem, and poses a great threat to human health. Especially, Listeria monocytogenes and Staphylococcus aureus are gram-positive bacteria found on food-contact surfaces with biofilms. These foodborne pathogens cause a considerable number of food poisoning and infections annually. Ovomucin (OM) is a water-insoluble gel-type glycoprotein in egg whites. Enzymatic hydrolysis can be used to improve the bioactive properties of OM. This study aimed to investigate whether ovomucin hydrolysates (OMHs) produced using five commercial enzymes (Alcalase®, Bromelain, α-Chymotrypsin, Papain, and Pancreatin) can inhibit the biofilm formation of L. monocytogenes ATCC 15313, L. monocytogenes H7962, S. aureus KCCM 11593, and S. aureus 7. Particularly, OMH prepared with papain (OMPP; 500 μg/mL) significantly inhibited biofilm formation in L. monocytogenes ATCC 15313, L. monocytogenes H7962, S. aureus KCCM 11593, and S. aureus 7 by 85.56 %, 80.28 %, 91.70 %, and 79.00 %, respectively. In addition, OMPP reduced the metabolic activity, exopolysaccharide production (EPS), adhesion ability, and gene expression associated with the biofilm formation of these bacterial strains. These results suggest that OMH, especially OMPP, exerts anti-biofilm effects against L. monocytogenes and S. aureus. Therefore, OMPP can be used as a natural anti-biofilm agent to control food poisoning in the food industry.