Abstract:
Ovomucin (OM), which has insoluble fractions is a viscous glycoprotein, found in egg albumin. Enzymatic hydrolysates of OM have water solubility and bioactive properties. This study investigated that the immunostimulatory effects of OM hydrolysates (OMHs) obtained by using various proteolytic enzymes (Alcalase®, bromelain, α-chymotrypsin, Neutrase®, pancreatin, papain, Protamax®, and trypsin) in RAW 264.7 cells. The results showed that OMH prepared with pancreatin (OMPA) produced the highest levels of nitrite oxide in RAW 264.7 cells, through upregulation of inducible nitric oxide synthase mRNA expression. The production of pro-inflammatory cytokines such as tumor necrosis factor-α and interleukin-6 were increased with the cytokines mRNA expression. The effect of OMPA on mitogen-activated protein kinase signaling pathway was increased the phosphorylation of p38, c-Jun NH2-terminal kinase, and extracellular signal-regulated kinase in a concentration-dependent manner. Therefore, OMPA could be used as a potential immune-stimulating agent in the functional food industry.
摘要:
Ovolucin(OM),具有不溶部分的是粘性糖蛋白,在鸡蛋白蛋白中发现。OM的酶解产物具有水溶性和生物活性。本研究调查了通过使用各种蛋白水解酶(Alcalase®,菠萝蛋白酶,α-胰凝乳蛋白酶,Neutrase®,胰酶,木瓜蛋白酶,Protamax®,和胰蛋白酶)在RAW264.7细胞中。结果表明,用胰酶(OMPA)制备的OMH在RAW264.7细胞中产生的亚硝酸盐氧化物水平最高,通过上调诱导型一氧化氮合酶mRNA表达。促炎细胞因子如肿瘤坏死因子-α和白细胞介素-6的产生随着细胞因子mRNA的表达而增加。OMPA对丝裂原活化蛋白激酶信号通路的影响是p38,c-JunNH2末端激酶磷酸化增加,和细胞外信号调节激酶以浓度依赖的方式。因此,OMPA可以在功能性食品工业中用作潜在的免疫刺激剂。