Abstract：
Essential oils show several biological properties, such as antimicrobial activity, but have limitations regarding their availability and stability. To maximize their antimicrobial effect and protection against environmental conditions, Pickering-type emulsions were used to vehiculate oregano essential oil (OEO) using cellulose nanofibers (CNF) as emulsion stabilizer. Enzymatic hydrolysis was used to produce CNF from a food industry waste (cassava peel), obtaining an environmentally sustainable emulsion stabilizer. It was evaluated how the different properties of the nanofibers affected the stability of the emulsions. Furthermore, the composition of the dispersed phase was varied (different ratios of OEO and sunflower oil-SO) in view of the target application in biodegradable active coatings. Even at very low concentration (0.01 % w/w), CNF was able to form kinetically stable emulsions with small droplet sizes using oil mixtures (OEO + SO). The stabilization mechanism was not purely Pickering, as there was a reduction in interfacial tension. Excellent antimicrobial activity was observed against bacteria and the fungus Alternaria alternata, demonstrating the ability to apply these emulsions in active systems such as coatings and films. An improvement in the stability of emulsions was observed when using a mixture of oils, which is extremely advantageous considering costs and stability to heat treatments, since the desired antimicrobial activity is maintained for the final application.
摘要：
精油显示出几种生物学特性，如抗菌活性，但对其可用性和稳定性有限制。为了最大限度地提高其抗菌效果和对环境条件的保护，Pickering型乳液用于以纤维素纳米纤维（CNF）为乳液稳定剂的牛至精油（OEO）。酶水解用于从食品工业废物（木薯皮）生产CNF，获得环境可持续的乳液稳定剂。评价了纳米纤维的不同性质如何影响乳液的稳定性。此外，鉴于在可生物降解的活性涂料中的目标应用，分散相的组成有所不同（OEO和葵花籽油-SO的比例不同）。即使在非常低的浓度（0.01％w/w），CNF能够使用油混合物(OEO+SO)形成具有小液滴尺寸的动力学稳定乳液。稳定机制不是纯粹的皮克林，因为界面张力降低了。对细菌和真菌链格孢菌观察到优异的抗菌活性，证明了将这些乳液应用于活性体系如涂层和薄膜的能力。当使用油的混合物时，观察到乳液稳定性的改善。考虑到热处理的成本和稳定性，这是非常有利的，因为在最终应用中保持了所需的抗微生物活性。
