Abstract：
The aim of this work was to develop, for the first time, sustainable strategies, based on the use of Ultrasound-Assisted Extraction, Natural Deep Eutectic Solvents, and Pressurized Liquid Extraction, to extract proteins from lime (Citrus x latifolia) peels and to evaluate their potential to release bioactive peptides. PLE showed the largest extraction of proteins (66-69%), which were hydrolysed using three different enzymes (Alcalase 2.4 L FG, Alcalase®PURE 2.4 L, and Thermolysin). The in vitro antioxidant and antihypertensive activities of released peptides were evaluated. Although all hydrolysates showed antioxidant and antihypertensive activity, the hydrolysate obtained with Thermolysin showed the most significant values. Since the Total Phenolic Content in all hydrolysates was low, peptides were likely the main contributors to these bioactivities. Hydrolysates were analyzed by UHPLC-QTOF-MS and a total of 98 different peptides were identified. Most of these peptides were rich in amino acids associated with antioxidant activity.
摘要：
这项工作的目的是发展，第一次,可持续战略,基于超声辅助提取的使用，天然深共晶溶剂,和加压液体萃取，从石灰（柑橘xlatifolia）果皮中提取蛋白质，并评估其释放生物活性肽的潜力。PLE显示最大的蛋白质提取（66-69％），使用三种不同的酶水解(Alcalase2.4LFG，Alcalase®PURE2.4L,和嗜热菌蛋白酶）。评价了释放肽的体外抗氧化和降压活性。尽管所有水解产物都显示出抗氧化和降压活性，用嗜热菌蛋白酶获得的水解产物显示出最显著的值。由于所有水解产物中的总酚类含量较低，肽可能是这些生物活性的主要贡献者。通过UHPLC-QTOF-MS分析水解产物,鉴定出总共98种不同的肽。这些肽中的大多数富含与抗氧化活性相关的氨基酸。
