未经批准:家畜和野生动物是抗生素抗性细菌的重要储库。本研究旨在在Salaphra野生动物保护区的农业用地界面区域从家畜和野生动物的粪便中分离大肠杆菌,泰国,并研究这些分离株的系统发育特性和抗生素耐药性。
未经评估:在此横截面中,描述性研究,我们从自由放养的野生动物(鹿和大象)和家畜(牛和山羊)中随机收集地面粪便。将所有粪便样品接种到MacConkey琼脂平板上,将乳糖发酵菌落鉴定为大肠杆菌。使用圆盘扩散法测定大肠杆菌分离株的抗生素敏感性。聚合酶链反应测定用于检测抗生素抗性和毒力基因。
UNASSIGNED:我们从收集的粪便样品中获得了362种大肠杆菌分离物。根据毒力基因将大肠杆菌分离株分为四个系统发育组(chuA,vjaA,和TspE4C2)。系统发育D组以鹿(41.67%)和大象(63.29%)为主,而系统发育组B1在牛中占主导地位(62.31%),山羊中系统发育组A(36.36%)和B2(33.33%)占主导地位。抗生素药敏试验显示,大多数耐抗生素的大肠杆菌是从国内山羊中分离出的(96.96%)。在362个大肠杆菌分离物中,38(10.5%)对至少一种抗生素具有抗性,21例(5.8%)对两种抗生素耐药,6例(1.66%)对3种或3种以上抗生素耐药。氨苄西林(AMP)是大肠杆菌耐药最常见的抗生素(48.48%),其次是四环素(TET)(45.45%)和甲氧苄啶-磺胺甲恶唑(3.03%)。从大象中分离出的一种对五种抗生素具有抗性:AMP,阿莫西林,硫化异恶唑,TET,还有环丙沙星.抗生素抗性基因的测定证实大肠杆菌分离株携带与对抗生素的表型抗性相关的抗生素抗性基因。大多数抗生素抗性大肠杆菌属于系统发育组A和B1,大多数非抗性大肠杆菌属于系统发育组B2和D。
UNASSIGNED:监测来自野生和家畜的大肠杆菌分离株表明,所有四个系统发育组的大肠杆菌都产生了抗生素耐药性,是多药耐药性的潜在来源。高水平的抗生素耐药性与家畜有关。我们的结果支持加强监测,以监测动物中抗生素抗性微生物的出现和影响。
UNASSIGNED: Domestic and wild animals are important reservoirs for antibiotic-resistant bacteria. This study aimed to isolate Escherichia coli from feces of domestic and wild animals at an agricultural land interface area of Salaphra Wildlife Sanctuary, Thailand, and study the phylogenic characteristics and antibiotic resistance in these isolates.
UNASSIGNED: In this cross-sectional, descriptive study, we randomly collected ground feces from free-ranging wild animals (deer and elephants) and domestic animals (cattle and goats). All fecal samples were inoculated onto MacConkey agar plates, and lactose-fermenting colonies were identified as E. coli. Antibiotic susceptibility of the E. coli isolates was determined using the disc diffusion method. Polymerase chain reaction assays were used to detect antibiotic resistance and virulence genes.
UNASSIGNED: We obtained 362 E. coli isolates from the collected fecal samples. The E. coli isolates were categorized into four phylogenetic groups according to the virulence genes (chuA, vjaA, and TspE4C2). Phylogenetic Group D was predominant in the deer (41.67%) and elephants (63.29%), whereas phylogenetic Group B1 was predominant in the cattle (62.31%), and phylogenetic Groups A (36.36%) and B2 (33.33%) were predominant in the goats. Antibiotic susceptibility testing revealed that most antibiotic-resistant E. coli were isolated from domestic goats (96.96%). Among the 362 E. coli isolates, 38 (10.5%) were resistant to at least one antibiotic, 21 (5.8%) were resistant to two antibiotics, and 6 (1.66%) were resistant to three or more antibiotics. Ampicillin (AMP) was the most common antibiotic (48.48%) to which the E. coli were resistant, followed by tetracycline (TET) (45.45%) and trimethoprim-sulfamethoxazole (3.03%). One isolate from an elephant was resistant to five antibiotics: AMP, amoxicillin, sulfisoxazole, TET, and ciprofloxacin. Determination of antibiotic resistance genes confirmed that E. coli isolates carried antibiotic resistance genes associated with phenotypic resistance to antibiotics. Most antibiotic-resistant E. coli belonged to phylogenic Groups A and B1, and most non-resistant E. coli belonged to phylogenic Groups B2 and D.
UNASSIGNED: Monitoring E. coli isolates from wild and domestic animals showed that all four phylogenic groups of E. coli have developed antibiotic resistance and are potential sources of multidrug resistance. High levels of antibiotic resistance have been linked to domestic animals. Our results support strengthening surveillance to monitor the emergence and effects of antibiotic-resistant microorganisms in animals.