一个2.5岁的人,25.5kg,撒尿的雌性澳大利亚牧羊犬有2个月的四肢腿部跛行移位史,四肢轻瘫,进行性嗜睡,和剧烈的疼痛。在体检中,发烧(40.61°C),心动过速,呼吸急促,轻度弥漫性骨盆肢体肌萎缩,观察到左cap前和右pop淋巴结肿大。由于预后不良和疼痛管理困难,人道安乐死当选。宏观和组织学发现显示多病灶合并肉芽肿,右股骨中央溶血性坏死区域,左肱骨,左肩胛骨,左肱二头肌,右半膜肌,肝脏,脾,脾和淋巴结。坏死区域包含无数的病灶内,细胞内,和细胞外阴性染色,无色素,间隔锐角分支菌丝,平行壁宽度为3-6μm,极性球形突起宽度为7-13μm。将肝脏的新鲜样品进行真菌培养。针对主要保守基因ITS的泛真菌PCR,浴缸,经CAL确认的福氏拟青霉。拟青霉属。是分类为子囊门的变形真菌的成员。拟青霉病是由拟青霉属引起的罕见真菌感染,在人类和动物中报道的疾病从浅表到全身临床形式,影响免疫功能低下和免疫功能正常的个体。在狗中,已经报道了播散性拟青霉病,但是真菌的种类并不总是确定的。据我们所知,这是犬中首例由Formosus感染引起的播散性拟霉菌病。
A 2.5-year-old, 25.5 kg, spayed female Australian Shepherd dog had a 2-month history of shifting leg lameness in all limbs, tetraparesis, progressive lethargy, and severe pain. On the physical examination, fever (40.61°C), tachycardia, tachypnea, mild diffuse pelvic limb muscular atrophy, left prescapular and right popliteal lymphadenomegaly were observed. Due to the poor prognosis and difficult pain management, humane euthanasia was elected. Macroscopic and histological findings revealed multifocal to coalescing granulomas with central areas of lytic necrosis within the right femur, left humerus, left scapula, left biceps brachii, right semimembranosus muscle, liver, spleen, and lymph nodes. The necrotic areas contained myriad intralesional, intracellular, and extracellular negatively stained, non-pigmented, septate acute angle branching hyphae with parallel walls measuring 3-6 μm in width with polar bulbous projections measuring 7-13 μm in width. Fresh samples of the liver were submitted for fungal culture. Panfungal PCR targeting the major conserved genes-ITS, TUB, CAL-confirmed Paecilomyces formosus. Paecilomyces spp. are members of anamorphic fungi classified under the phylum Ascomycota. Paecilomycosis is an uncommon fungal infection caused by Paecilomyces spp with a disease reported in humans and animals ranging from superficial to systemic clinical forms affecting both immunocompromised and immunocompetent individuals. In dogs, disseminated paecilomycosis has been reported, but the species of fungi are not always determined. To our knowledge, this is the first
case of disseminated paecilomycosis caused by P. formosus infection in a dog.