Abstract：
Blastocystis is an enteric protozoan able to infect humans and a large variety of animal hosts worldwide. It exhibits significant genetic diversity, with at least 17 subtypes (STs) identified to date, most of which have low host specificity. In the present study, a cross-sectional survey was conducted on Blastocystis infection among humans and domestic animals sharing habitats in northeastern China\'s Heilongjiang Province, in order to investigate the frequency and subtype distribution and to evaluate the risk of the zoonotic transmission of Blastocystis sp..Results show that the frequency was 10.5% (6/57), and three subtypes (ST1, ST2, and ST3) were found in humans; in animals, the frequency was 17.9% (46/257), and six subtypes (ST1, ST3, ST5, ST7, ST10, and ST26) were detected. A significant association between Blastocystis sp. infection and eating unwashed vegetables and fruits was found (P = 0.007). We found no effect on gender, age and season on Blastocystis sp. colonization. Phylogenetic analysis showed that Blastocystis ST7 sequences from chicken in two geographical locations formed two distinct clades. Alleles were identified using the Blastocystis 18S database, and a total of 10 different alleles were found in seven STs. Overlap of STs in humans and domestic animals (pig or cattle) was observed in Dongfanghong village. The findings of potentially zoonotic subtypes in domestic animals suggest that these animals may serve as reservoirs of human Blastocystis sp. infections. Multisectoral cooperation is needed to slow down the transmission of Blastocystis in domestic animals, minimize environmental contamination by Blastocystis cysts, and increase molecular epidemiological surveillance of Blastocystis sp. in humans and animals.
摘要：
囊胚是一种肠道原生动物，能够感染人类和全世界各种各样的动物宿主。它表现出显著的遗传多样性，迄今为止，至少确定了17种亚型(STs)，其中大多数具有低宿主特异性。在本研究中,在中国东北黑龙江省，对人类和家畜共享栖息地的囊胚原虫感染进行了横断面调查，为了研究囊胚的频率和亚型分布并评估人畜共患病传播的风险。.结果表明，频率为10.5%(6/57)，在人类中发现了三种亚型（ST1，ST2和ST3）；在动物中，频率为17.9%(46/257)，检测到6种亚型(ST1、ST3、ST5、ST7、ST10和ST26)。囊胚菌之间存在显着关联。发现感染和食用未清洗的蔬菜和水果（P=0.007）。我们发现对性别没有影响，囊胚的年龄和季节。殖民。系统发育分析表明，来自两个地理位置的鸡的囊胚ST7序列形成了两个不同的进化枝。使用囊胚18S数据库鉴定等位基因，在7个STs中发现了10个不同的等位基因。在东方红村观察到人和家畜（猪或牛）中的STs重叠。家养动物中潜在的人畜共患亚型的发现表明，这些动物可能是人类胚泡的储库。感染。需要多部门合作来减缓囊胚原虫在家畜中的传播,尽量减少囊胚囊肿对环境的污染，并增加囊胚菌的分子流行病学监测。在人类和动物中。
