牙龈卟啉单胞菌与牙周炎的进展有关,以牙周组织的炎症和破坏为特征。这里,我们报告说抹茶,山茶的产品,通过多种策略阻碍牙龈卟啉单胞菌的坚持和存活。抹茶提取物(ME)不仅抑制了牙龈卟啉单胞菌的生长,而且还抑制了黑质普氏菌和核梭杆菌的生长,虽然它没有抑制9种口腔链球菌和Aggregatibacter放线菌的生长。ME介导的牙龈卟啉单胞菌生长抑制的特征在于细菌包膜的形态和生理变化。其伴随着纳米颗粒的形成和降低的膜流动性/渗透性而不损失膜的完整性。ME还以主要菌毛(FimA)依赖性方式触发牙龈卟啉单胞菌的自动聚集。此外,牙龈卟啉单胞菌的粘附被ME显著抑制,不管菌毛。此外,一项结构-活性关系研究测试了从ME中分离的一系列儿茶素,并确定儿茶素的邻苯三酚型B环对于抑制牙龈卟啉生长是必需的。在一项临床研究中,评估抹茶漱口水对牙周炎患者的微生物学和治疗效果,与干预前水平相比,抹茶漱口水显著降低了唾液中牙龈卟啉单胞菌的数量。在抹茶组中观察到探查口袋深度有改善的趋势,尽管差异无统计学意义。一起来看,我们提出了一个概念证明,基于抹茶对牙龈卟啉单胞菌的多模式抑制作用,抹茶可能对牙周炎的预防和治疗具有临床适用性。
目的:牙周炎,一种多因素的口腔炎症性疾病,导致牙槽骨破坏,是人类牙齿脱落的主要原因。此外,新出现的证据表明,牙周炎和广泛的其他慢性炎症驱动的疾病之间存在关联,包括糖尿病,早产,心血管疾病,吸入性肺炎,类风湿性关节炎,认知障碍,和癌症。在本研究中,我们报告说抹茶,山茶的产品,牙龈卟啉单胞菌,一种主要的牙周病菌,不仅在一系列体外实验中,而且在牙周炎患者的试点干预临床试验中,其中抹茶漱口水在统计学上显着减少唾液中的牙龈卟啉单胞菌数量,与干预前水平相比。一起来看,我们认为抹茶对牙周炎的预防和治疗可能具有临床适用性。
Porphyromonas gingivalis has been associated with progression of periodontitis, characterized by inflammation and destruction of periodontal tissues. Here, we report that matcha, a product of Camellia sinensis, hampers the adherence and survival of P. gingivalis through multiple tactics. Matcha extract (ME) inhibited the growth not only of P. gingivalis but also of Prevotella nigrescens and Fusobacterium nucleatum, while it did not inhibit growth of nine species of oral streptococci and Aggregatibacter actinomycetemcomitans. ME-mediated P. gingivalis growth inhibition was characterized by both morphological and physiological changes at the bacterial envelope, which were accompanied by nano-particle formation and decreased membrane fluidity/permeability without loss of membrane integrity. ME also triggered autoaggregation of P. gingivalis in a major fimbriae (FimA)-dependent manner. In addition, adherence of P. gingivalis was dramatically inhibited by ME, irrespective of fimbriae. Furthermore, a structure-activity relationship study tested a series of
catechins isolated from ME and identified the pyrogallol-type B-ring of
catechins as essential for P. gingivalis growth inhibition. In a clinical study to assess the microbiological and therapeutic effects of matcha mouthwash in patients with periodontitis, the P. gingivalis number in saliva was significantly reduced by matcha mouthwash compared to the pre-intervention level. A tendency toward improvement in probing pocket depth was observed in the matcha group, although the difference was not statistically significant. Taken together, we present a proof of concept, based on the multimodal inhibitory effect of matcha against P. gingivalis, and that matcha may have clinical applicability for prevention and treatment of periodontitis.
OBJECTIVE: Periodontitis, a multifactorial inflammatory disease of the oral cavity, results in alveolar bone destruction, and is a major cause of tooth loss of humans. In addition, emerging evidence has demonstrated associations between periodontitis and a wide range of other chronic inflammation-driven disorders, including diabetes mellitus, preterm birth, cardiovascular disease, aspiration pneumonia, rheumatoid arthritis, cognitive disorder, and cancer. In the present study, we report that matcha, a product of Camellia sinensis, hampers Porphyromonas gingivalis, a major periodontal pathobiont, in not only a series of in vitro experiments but also a pilot intervention clinical trial of patients with periodontitis, in which matcha mouthwash statistically significantly reduced the P. gingivalis number in saliva, as compared to the pre-intervention level. Taken together, we suggest that matcha may have clinical applicability for prevention and treatment of periodontitis.