Abstract：
Tea drinking impacts aging and aging-related diseases. However, knowledge of anti-aging molecules other than the major catechins in complex tea extracts remains limited. Here we used Caenorhabditis elegans to analyze the longevity effects of tea extracts and constituents comprehensively. We found that the hot water extract of green tea prolonged lifespan and heathspan. Further, the MeOH fraction prolonged lifespan significantly longer than other fractions. Correlation analysis between mass spectroscopic data and anti-aging activity suggests that ester-type catechins (ETCs) are the major anti-aging components, including 4 common ETCs, 6 phenylpropanoid-substituted ester-type catechins (PSECs), 5 cinnamoylated catechins (CCs), 7 ester-type flavoalkaloids (ETFs), and 4 cinnamoylated flavoalkaloids (CFs). CFs (200 μM) are the strongest anti-aging ETCs (with the longest 73% lifespan extension). Green tea hot water extracts and ETCs improved healthspan by enhancing stress resistance and reducing ROS accumulation. The mechanistic study suggests that they work by multiple pathways. Moreover, ETCs modulated gut microbial homeostasis, increased the content of short-chain fatty acids, and reduced fat content. Altogether, our study provides new evidence for the anti-aging benefits of green tea and insights into a deep understanding of the chemical truth and multi-target mechanism.
摘要：
喝茶会影响衰老和衰老相关疾病。然而,除了复合茶提取物中的主要儿茶素以外的抗衰老分子的知识仍然有限。在这里，我们使用秀丽隐杆线虫对茶提取物和成分的长寿作用进行了全面分析。我们发现，绿茶的热水提取物延长了寿命和希思跨度。Further,MeOH级分的寿命明显长于其他级分。质谱数据与抗衰老活性的相关性分析表明,酯型儿茶素(ETCs)是抗衰老的主要成分,包括4种常见ETC，6苯丙素取代的酯型儿茶素（PSECs），5肉桂酰儿茶素（CC），7酯型黄体生物碱（ETF），和4种肉桂酰化的黄素生物碱(CFs)。CFs(200μM)是最强的抗老化ETC(具有最长的73%的寿命延长)。绿茶热水提取物和ETC通过增强抗逆性和减少ROS积累来改善健康状况。机械研究表明，它们通过多种途径起作用。此外，ETCs调节肠道微生物稳态，增加了短链脂肪酸的含量，减少脂肪含量。总之,我们的研究为绿茶的抗衰老益处提供了新的证据，并深入了解了对化学真相和多靶点机制的理解。
