Abstract：
Oxidative stress and inflammation caused by cisplatin, which is frequently used in the treatment of many cancers, damage healthy tissues as well as cancer cells. In this study, we aimed to investigate the effect of epigallocatechin-3-gallate (EGCG) and infliximab (INF) administration on pancreatic endocrine cells in rats treated with systemic cisplatin (CDDP). The rats were randomly divided into 6 groups: group 1 (control group), group 2 (EGCG group), group 3 (CDDP group), group 4 (EGCG + CDDP group), group 5 (CDDP + INF group), and group 6 (EGCG + CDDP + INF group). The study\'s findings demonstrated that EGCG and INF effectively reduced the cellular damage induced by CDDP in histopathologic investigations of the pancreas. EGCG and INF, whether used individually or in combination, demonstrated a significant reduction in malondialdehyde (MDA) levels and an increase in glutathione (GSH) levels in the rat pancreas compared to the CDDP group. Immunohistochemically, the enhanced presence of insulin and glucagon positivity in the EGCG and INF groups, along with the absence of TUNEL immunopositivity, indicate that both treatments reduced CDDP-induced apoptosis. Furthermore, the observed lack of immunopositivity in TNF-α and 8-OHdG in the groups treated with EGCG and INF, compared to those treated with CDDP, indicates that these substances can inhibit inflammation. EGCG and INF, whether provided alone or together, can potentially reduce the damage caused to pancreatic islet cells by cisplatin. This effect is achieved through their anti-inflammatory and antioxidant properties during the early stages of the condition.
摘要：
由顺铂引起的氧化应激和炎症，它经常用于治疗许多癌症，损伤健康组织以及癌细胞。在这项研究中,我们旨在研究表没食子儿茶素没食子酸酯(EGCG)和英夫利昔单抗(INF)对系统性顺铂(CDDP)治疗大鼠胰腺内分泌细胞的影响.将大鼠随机分为6组:第1组(对照组),第2组（EGCG组），第3组（CDDP组），第4组（EGCG+CDDP组），第5组（CDDP+INF组），第6组(EGCG+CDDP+INF组)。研究结果表明，EGCG和INF在胰腺组织病理学检查中可有效降低CDDP引起的细胞损伤。EGCG和INF，无论是单独使用还是组合使用，与CDDP组相比，大鼠胰腺中丙二醛（MDA）水平显着降低，谷胱甘肽（GSH）水平升高。免疫组织化学，EGCG和INF组中胰岛素和胰高血糖素阳性的存在增强，除了没有TUNEL免疫阳性，表明两种治疗均减少了CDDP诱导的细胞凋亡。此外，在用EGCG和INF治疗的组中观察到TNF-α和8-OHdG缺乏免疫阳性，与CDDP治疗的患者相比，表明这些物质可以抑制炎症。EGCG和INF，无论是单独提供还是一起提供，可以潜在地减少顺铂对胰岛细胞造成的损伤。这种效果是通过其在疾病早期阶段的抗炎和抗氧化特性来实现的。
