■最近,使用植物药作为抗球虫药的替代品一直是控制球虫病的一种有吸引力的方法。因此,进行这项研究是为了评估克拉美草(根)(KLRE)的甲醇水提取物(200mg/kg)对乳头艾美球虫引起的感染的潜在作用。
■总共25只雄性C57BL/6小鼠分为五组(I,II,III,IV,和V)。在实验的第一天,除I组(对照组)和II组(未经KLRE感染的治疗组)外,用103个孢子化的乳头虫卵囊口服接种。在感染当天,IV组采用KLRE治疗。第V组作为感染治疗组,用安普林(抗球虫药)治疗。
■用提取物和抗球虫药治疗连续五天。虽然没有达到参考药物(安普林)的疗效水平,根据关键标准评估,KLRE表现出显著的抗球虫活性,包括卵囊抑制率,总寄生阶段,和维持营养稳态。KLRE中酚类和类黄酮化合物的存在被认为是其积极作用的原因。艾美球虫感染增加了空肠的氧化损伤。KLRE处理显著提高了过氧化氢酶和超氧化物歧化酶的活性。相反,KLRE降低了丙二醛和一氧化氮的水平。此外,KLRE处理减少小鼠空肠组织中的巨噬细胞浸润,以及CD4T细胞和NFkB的程度。如诱导型一氧化氮合酶(iNOs)-mRNA的上调所揭示的,乳头大肠杆菌引起全身性炎症反应的状态。经KLRE治疗,iNOs的活性从3.63倍降低到1.46倍。此外,KLRE能够下调促炎细胞因子干扰素-γ的表达,核因子κB,和白细胞介素-10-mRNA的1.63、1.64和1.38倍,分别。此外,KLRE显示IL-10蛋白表达水平从104.27±8.41pg/ml显著降低至62.18±3.63pg/ml。
■集体,K.lappacea是一种有前途的草药,可以改善空肠的氧化应激和炎症,在小鼠中由乳头虫感染诱导。
UNASSIGNED: Recently, the use of botanicals as an alternative to coccidiostats has been an appealing approach for controlling coccidiosis. Therefore, this study was conducted to evaluate the potential role of aqueous methanolic extract (200 mg/kg) of Krameria lappacea (roots) (KLRE) against infection induced by Eimeria papillata.
UNASSIGNED: A total of 25 male C57BL/6 mice were divided into five groups (I, II, III, IV, and V). On 1st day of the experiment, all groups except groups I (control) and II (non-infected-treated group with KLRE), were inoculated orally with 103 sporulated E. papillata oocysts. On the day of infection, group IV was treated with KLRE. Group V served as an infected-treated group and was treated with amprolium (coccidiostat).
UNASSIGNED: Treatment with extract and coccidiostat was continued for five consecutive days. While not reaching the efficacy level of the reference drug (amprolium), KLRE exhibited notable anticoccidial activity as assessed by key criteria, including oocyst suppression rate, total parasitic stages, and maintenance of nutrient homeostasis. The presence of phenolic and flavonoid compounds in KLRE is thought to be responsible for its positive effects. The Eimeria infection increased the oxidative damage in the jejunum. KLRE treatment significantly increased the activity of catalase and superoxide dismutase. On the contrary, KLRE decreased the level of malondialdehyde and nitric oxide. Moreover, KLRE treatment decreased macrophage infiltration in the mice jejunal tissue, as well as the extent of CD4 T cells and NFkB. E. papillata caused a state of systemic inflammatory response as revealed by the upregulation of inducible nitric oxide synthase (iNOs)-mRNA. Upon treatment with KLRE, the activity of iNOs was reduced from 3.63 to 1.46 fold. Moreover, KLRE was able to downregulate the expression of pro-inflammatory cytokines interferon-γ, nuclear factor kappa B, and interleukin-10 -mRNA by 1.63, 1.64, and 1.38 fold, respectively. Moreover, KLRE showed a significant reduction in the expression of IL-10 protein level from 104.27 ± 8.41 pg/ml to 62.18 ± 3.63 pg/ml.
UNASSIGNED: Collectively, K. lappacea is a promising herbal medicine that could ameliorate the oxidative stress and inflammation of jejunum, induced by E. papillata infection in mice.