Abstract：
The development of an effective humoral response to pathogens and immunogens is a multiphase biological process, which is mediated by the coordinated function of specialized immune cell types in secondary lymphoid organs and particularly in T cell and follicular areas. More specifically, within the follicular/germinal center area, the orchestrated interplay between B cells, follicular helper CD4 T cells (Tfh), and stromal cells triggers a cascade of immune reactions leading to the development of memory B cells and plasma cells able to generate effective, antigen-specific antibodies. The role of Tfh cells in this process is critical. Given the need for vaccines capable to induce antibodies of high affinity, neutralizing activity, and durability, understanding the cellular and molecular mechanisms regulating Tfh cell development is of great importance. Here, we describe novel approaches for the comprehensive understanding of these cells and possible implications for future studies in vaccine development and the understanding of the pathogenesis of relevant diseases.
摘要：
对病原体和免疫原的有效体液应答的发展是一个多阶段的生物学过程,这是由次级淋巴器官，特别是T细胞和滤泡区的特殊免疫细胞类型的协调功能介导的。更具体地说,在卵泡/生发中心区域内，B细胞之间精心策划的相互作用，滤泡辅助性CD4T细胞（Tfh），和基质细胞触发一系列免疫反应，导致记忆B细胞和浆细胞的发育能够产生有效的，抗原特异性抗体。Tfh细胞在这一过程中的作用是至关重要的。鉴于需要能够诱导高亲和力抗体的疫苗，中和活性，和耐用性，了解调节Tfh细胞发育的细胞和分子机制具有重要意义。这里,我们描述了全面了解这些细胞的新方法，以及对疫苗开发和相关疾病发病机理的理解的未来研究的可能意义。
