%0 Journal Article
%T Oleic acid enhances proliferation and calcium mobilization of CD3/CD28 activated CD4+ T cells through incorporation into membrane lipids.
%A von Hegedus JH
%A de Jong AJ
%A Hoekstra AT
%A Spronsen E
%A Zhu W
%A Cabukusta B
%A Kwekkeboom JC
%A Heijink M
%A Bos E
%A Berkers CR
%A Giera MA
%A Toes REM
%A Ioan-Facsinay A
%J Eur J Immunol
%V 0
%N 0
%D 2024 Jun 18
%M 38890809
%F 6.688
%R 10.1002/eji.202350685
%X Unsaturated fatty acids (UFA) are crucial for T-cell effector functions, as they can affect the growth, differentiation, survival, and function of T cells. Nonetheless, the mechanisms by which UFA affects T-cell behavior are ill-defined. Therefore, we analyzed the processing of oleic acid, a prominent UFA abundantly present in blood, adipocytes, and the fat pads surrounding lymph nodes, in CD4+ T cells. We found that exogenous oleic acid increases proliferation and enhances the calcium flux response upon CD3/CD28 activation. By using a variety of techniques, we found that the incorporation of oleic acid into membrane lipids, rather than regulation of cellular metabolism or TCR expression, is essential for its effects on CD4+ T cells. These results provide novel insights into the mechanism through which exogenous oleic acid enhances CD4+ T-cell function.