目标:2004年,食用天使翅蘑菇后,胸膜囊孢原,日本报告了59起食物中毒事件。因此,17人死于急性脑病。2023年,我们证明了凝集素,胸膜环胺,这种蘑菇的胸膜环氮吡啶对小鼠的大脑造成了损害。尽管我们在2013年报道了P.porrigens的基因组和转录组数据,但转录组数据的装配质量不足以进行准确的功能注释。因此,我们使用IlluminaNovaSeq6000获得了有关该蘑菇子实体和菌丝体的详细转录组数据。
结果:从头组装数据表明,子实体和菌丝体的N50长度与以前报道的相比有所改善。子实体和菌丝体之间的差异表达分析表明,子实体和菌丝体中1,937和1,555个基因显着上调,分别。P.porrigens转录本的生物学功能,包括PA生物合成途径,使用BLAST搜索进行调查,基因本体论,和京都百科全书的基因和基因组途径分析。获得的结果显示L-缬氨酸,PA的预测前兆,在子实体和菌丝体中生物合成。此外,进行实时RT-PCR以评估差异表达分析结果的准确性。
OBJECTIVE: In 2004, after consuming angel-wing mushrooms, Pleurocybella porrigens, 59 incidents of food poisoning were reported in Japan. Consequently, 17 individuals died of acute encephalopathy. In 2023, we proved that a lectin, pleurocybelline, and pleurocybellaziridine from this mushroom caused damage to the brains of mice. Although we reported genomic and transcriptomic data of P. porrigens in 2013, the assembly quality of the transcriptomic data was inadequate for accurate functional annotation. Thus, we obtained detailed transcriptomic data on the fruiting bodies and mycelia of this mushroom using Illumina NovaSeq 6000.
RESULTS: De novo assembly data indicated that the N50 lengths for the fruiting bodies and mycelia were improved compared with those previously reported. The differential expression analysis between the fruiting bodies and the mycelia revealed that 1,937 and 1,555 genes were significantly up-regulated in the fruiting bodies and the mycelia, respectively. The biological functions of P. porrigens transcripts, including PA biosynthetic pathways, were investigated using BLAST search, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes pathway analysis. The obtained results revealed L-valine, a predicted precursor of PA, is biosynthesized in the fruiting bodies and mycelia. Furthermore, real-time RT-PCR was performed to evaluate the accuracy of the results of differential expression analysis.