{Reference Type}: Journal Article
{Title}: Qualitative real-time RT-PCR assay for nOPV2 poliovirus detection.
{Author}: Dolgova AS;Kanaeva OI;Antonov SA;Shabalina AV;Klyuchnikova EO;Sbarzaglia VA;Gladkikh AS;Ivanova OE;Kozlovskaya LI;Dedkov VG;
{Journal}: J Virol Methods
{Volume}: 329
{Issue}: 0
{Year}: 2024 Jun 15
{Factor}: 2.623
{DOI}: 10.1016/j.jviromet.2024.114984
{Abstract}: Based on the success of the Sabin2-based vaccine, a next-generation nOPV2 poliovirus vaccine has been developed. For epidemic monitoring and conducting epidemiological investigations, it is necessary to have a diagnostic assay with the ability to differentiate this variant from others. Here we describe such a real-time RT-PCR assay. The region with the cre insertion in the 5'-UTR was chosen as the target, and the limit of detection was 103 copies/mL (2.5×103 copies/mL using Probit analysis) determined using armored RNA particles. Sensitivity and specificity were 86.28 - 100 % and 76.84 - 100 %, respectively (with 95 % CI). Thus, this method can be effectively used when it is necessary to make a differential diagnosis of poliovirus strains.