Abstract：
Following the relaxation of COVID-19 restrictions, other respiratory viruses such as influenza and respiratory syncytial virus (RSV), whose transmission were decreased due to COVID-19 precautions, are rising again. Because of similar clinical features and reported co-infections, multiplex detection of SARS-CoV-2, influenza A/B, and RSV is required to use specific treatments. This study assessed an extraction-free sample preparation (heat treatment at 95°C for 3 minutes) for multiplex detection using rRT-PCR. Despite an observed Ct-delay (∆Ct) averageing 1.26 compared to the standard method, an acceptable total sensitivity of 92 % and a negative predictive value (NPV) of 96 % were obtained. Moreover, Implementation on a microfluidic chip demonstrated efficiency, maintaining an excellent correlation (R2=0.983) with the standard method. Combining this extraction-free procedure with rRT-PCR on a microfluidic chip seems promising, because it simplifies the design and reduces the cost and complexity of the integrated assay for multiplex detection of SARS-CoV-2, influenza A/B, and RSV.
摘要：
随着COVID-19限制的放松，其他呼吸道病毒，如流感和呼吸道合胞病毒（RSV），由于COVID-19的预防措施，其传播量减少，再次上升。由于相似的临床特征和报告的合并感染，SARS-CoV-2，甲型/乙型流感，和RSV需要使用特定的治疗。该研究评估了使用rRT-PCR进行多重检测的无提取样品制备(在95°C热处理3分钟)。尽管与标准方法相比，观察到的Ct延迟(ΔCt)平均为1.26，可接受的总灵敏度为92%,阴性预测值(NPV)为96%.此外，在微流控芯片上的实现证明了效率，与标准方法保持良好的相关性（R2=0.983）。将这种无提取程序与微流体芯片上的rRT-PCR相结合似乎很有希望，因为它简化了设计并降低了用于SARS-CoV-2，流感A/B多重检测的集成检测的成本和复杂性，RSV
