%0 Journal Article
%T Qualitative real-time RT-PCR assay for nOPV2 poliovirus detection.
%A Dolgova AS
%A Kanaeva OI
%A Antonov SA
%A Shabalina AV
%A Klyuchnikova EO
%A Sbarzaglia VA
%A Gladkikh AS
%A Ivanova OE
%A Kozlovskaya LI
%A Dedkov VG
%J J Virol Methods
%V 329
%N 0
%D 2024 Jun 15
%M 38885908
%F 2.623
%R 10.1016/j.jviromet.2024.114984
%X Based on the success of the Sabin2-based vaccine, a next-generation nOPV2 poliovirus vaccine has been developed. For epidemic monitoring and conducting epidemiological investigations, it is necessary to have a diagnostic assay with the ability to differentiate this variant from others. Here we describe such a real-time RT-PCR assay. The region with the cre insertion in the 5'-UTR was chosen as the target, and the limit of detection was 103 copies/mL (2.5×103 copies/mL using Probit analysis) determined using armored RNA particles. Sensitivity and specificity were 86.28 - 100 % and 76.84 - 100 %, respectively (with 95 % CI). Thus, this method can be effectively used when it is necessary to make a differential diagnosis of poliovirus strains.