%0 Journal Article
%T Real-Time RT-PCR for the Diagnosis of Epizootic Hemorrhagic Disease Virus.
%A Ashby M
%J Methods Mol Biol
%V 2838
%N 0
%D 2024
%M 39126630
暂无%R 10.1007/978-1-0716-4035-7_10
%X Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) has become an essential tool in rapid and reliable detection of animal diseases such as epizootic hemorrhagic disease (EHD). Here we provide a protocol for the detection of epizootic hemorrhagic disease virus (EHDV) genetic material in blood and tissue samples, using a real-time RT-PCR that targets a conserved region in segment 9 of the EHDV genome. This protocol can be used to detect up to approximately 90 samples in a single run and can be completed in less than 4 h.