Abstract：
Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) has become an essential tool in rapid and reliable detection of animal diseases such as epizootic hemorrhagic disease (EHD). Here we provide a protocol for the detection of epizootic hemorrhagic disease virus (EHDV) genetic material in blood and tissue samples, using a real-time RT-PCR that targets a conserved region in segment 9 of the EHDV genome. This protocol can be used to detect up to approximately 90 samples in a single run and can be completed in less than 4 h.
摘要：
实时逆转录聚合酶链反应（real-timeRT-PCR）已成为快速，可靠地检测动物疾病如流行病出血性疾病（EHD）的重要工具。在这里，我们提供了用于检测血液和组织样本中的流行病出血性疾病病毒（EHDV）遗传物质的方案，使用实时RT-PCR靶向EHDV基因组9段中的保守区域。该方案可用于在单次运行中检测多达约90个样品，并且可以在不到4小时内完成。
