翼状胬肉是最常见的眼部疾病之一,没有任何明确的病因。一些研究表明,阳光照射与翼状胬肉之间存在关联,但是其他人提出了遗传变异在其发病机理中的作用。迄今为止,没有研究调查炎症转录因子的相关性,中东翼状胬肉的NFκB基因。我们检测了翼状胬肉患者中3种炎症相关NFκB1,NFκB2和RELA基因的表达变化。本病例对照研究纳入了30例翼状胬肉患者和30例年龄和性别匹配的对照。没有参与者在其结膜中显示出任何炎症的临床迹象。获得了人口统计信息,并使用基因特异性引物通过实时RT-PCR在其结膜中测量了NFκB1,NFκB2和RELA三个基因的表达水平。患者NFκB1、NFκB2和RELA基因的平均表达水平分别比正常人高2.4±0.3、1.9±0.5和1.8±0.4倍,分别。在户外活动和阳光照射较多的个体中观察到较高水平的基因表达。此外,NFκB2和RELA基因的表达水平之间存在显着相关性,提示翼状胬肉患者可能形成NFκB2-RELA异二聚体。这项研究表明炎症相关的NFκB1、NFκB2和RELA基因的表达之间存在显着关联。还有翼状胬肉.进一步研究验证炎症在翼状胬肉发病机制中的作用,可能为翼状胬肉的管理提供新的目标。
Pterygium is one of the most common eye conditions without any clear etiology. Some studies have suggested an association between sun exposure and pterygium, but others have proposed the role of genetic variations in its pathogenesis. To date, no study has investigated the association of inflammatory transcription factor, NFκB genes with pterygium in the Middle East. We examined the changes in expression of 3 inflammatory related NFκB1, NFκB2, and RELA genes in patients with pterygium. Thirty patients with pterygium and 30 age and sex-matched controls were enrolled in this
case-control study. None of the participants showed any clinical signs of inflammation in their conjunctiva. Demographic information was obtained and the expression levels of three genes including NFκB1, NFκB2, and RELA were measured in their conjunctiva by real-time RT-PCR using gene-specific primers. Mean expression level of NFκB1, NFκB2 and RELA genes in patients were 2.4±0.3, 1.9± 0.5, and 1.8±0.4 times higher than normal subjects, respectively. Higher levels of gene expression were observed in individuals with more outdoor activity and sun exposure. Moreover, a significant correlation was observed between the expression levels of NFκB2 and RELA genes, suggesting a possible NFκB2- RELA heterodimer formation in patients with pterygium. This study has indicated a significant association between expressions of inflammatory-related NFκB1, NFκB2 and RELA genes, and pterygium. Further studies to verify the role of inflammation in the pathogenesis of pterygium, may provide new targets for managing pterygia.