三个放线菌菌株的分类位置,BCCO10_0061T,BCCO10_0798T,和BCCO10_0856T,从索科洛夫煤炭盆地的裸露土壤中回收,捷克共和国,是使用多相方法建立的。基于100个单拷贝基因的多位点序列分析将BCCO10_0061T定位在与Lentzeawayandensis相同的簇中,菌株BCCO10_0798T与Lentzeaflaviverrucosa相同,加州Lentzea,LentzeaViolacea,和Lentzeaalbidocapillata,菌株BCCO10_0856T与Lentzeakentuckyensis和Lentzeaalba聚集在一起。这些菌株的形态和化学分类学特征支持将其分配给Lentzea属。在所有三个菌株中,MK-9(H4)在类异戊二烯醌中占80%以上。细胞壁肽聚糖中的诊断二氨基酸是内消旋二氨基庚二酸。全细胞糖是鼠李糖,核糖,甘露糖,葡萄糖,还有半乳糖.主要脂肪酸(>10%)是异-C15:0、异-C15:0、异-C16:0和C16:0。极性脂质是二磷脂酰甘油,甲基磷脂酰乙醇胺,磷脂酰乙醇胺,羟基磷脂酰乙醇胺,磷脂酰甘油,和磷脂酰肌醇。BCCO10_0061T菌株的基因组DNAG+C含量(mol%)为68.8,69.2对于BCCO10_0798T,BCCO10_0856T为68.5。结合数字DNA-DNA杂交结果,BCCO10_0061T的平均核苷酸同一性值和表型特征,BCCO10_0798T,BCCO10_0856T将它们与密切相关的菌株区分开来。对菌株基因组序列的生物信息学分析揭示了几个生物合成基因簇(BGC),与已知簇的同一性>50%,包括用于地质蛋白的BGC,Coelichelin,ε-聚-1-赖氨酸,和红霉素样BGC。大多数鉴定的BGC与已知的BGC表现出低相似性(<50%),表明它们对于新的次级代谢产物的生物合成的遗传潜力。基于以上结果,每个菌株代表Lentzea属的一个新物种,为此,我们提出了Lentzeasokolovensissp。11月。对于BCCO10_0061T(=DSM116175T),Lentzeakristufekiisp.11月。对于BCCO10_0798T(=DSM116176T),和Lentzeamiocenicasp.11月。适用于BCCO10_0856T(=DSM116177T)。
The taxonomic position of three actinobacterial strains, BCCO 10_0061T, BCCO 10_0798T, and BCCO 10_0856T, recovered from bare soil in the Sokolov Coal Basin, Czech Republic, was established using a polyphasic approach. The multilocus sequence analysis based on 100 single-copy genes positioned BCCO 10_0061T in the same cluster as Lentzea waywayandensis, strain BCCO 10_0798T in the same cluster as Lentzea flaviverrucosa, Lentzea californiensis, Lentzea violacea, and Lentzea albidocapillata, and strain BCCO 10_0856T clustered together with Lentzea kentuckyensis and Lentzea alba. Morphological and chemotaxonomic characteristics of these strains support their assignment to the genus Lentzea. In all three strains, MK-9(H4) accounted for more than 80 % of the isoprenoid quinone. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The whole-cell sugars were rhamnose, ribose, mannose, glucose, and galactose. The major fatty acids (>10 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, and C16 : 0. The polar lipids were diphosphatidylglycerol, methyl-phosphatidylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol. The genomic DNA G+C content of strains (mol%) was 68.8 for BCCO 10_0061T, 69.2 for BCCO 10_0798T, and 68.5 for BCCO 10_0856T. The combination of digital DNA-DNA hybridization results, average nucleotide identity values and phenotypic characteristics of BCCO 10_0061T, BCCO 10_0798T, and BCCO 10_0856T distinguishes them from their closely related strains. Bioinformatic analysis of the genome sequences of the strains revealed several biosynthetic gene clusters (BGCs) with identities >50 % to already known clusters, including BGCs for geosmin, coelichelin, ε-poly-l-lysine, and erythromycin-like BGCs. Most of the identified BGCs showed low similarity to known BGCs (<50 %) suggesting their genetic potential for the biosynthesis of novel secondary metabolites. Based on the above results, each strain represents a novel species of the genus Lentzea, for which we propose the name Lentzea sokolovensis sp. nov. for BCCO 10_0061T (=DSM 116175T), Lentzea kristufekii sp. nov. for BCCO 10_0798T (=DSM 116176T), and Lentzea miocenica sp. nov. for BCCO 10_0856T (=DSM 116177T).