两个革兰氏染色阴性,杆状,不活动,需氧和产生类胡萝卜素的菌株,属于红杆菌科,指定为H149T和Z2T,从海南和浙江收集的滩涂沉积物样本中分离出来,中国公关,分别。菌株H149T的生长发生在15-42°C,0-10.0%(w/v)NaCl,和pH6.0-8.5,最佳温度为35-37°C,3.0-3.5%(w/v)NaCl和pH7.0。菌株Z2T在15-37°C生长,0-6.0%(w/v)NaCl,和pH6.0-9.5,最佳温度为25-30°C,0.5-1.0%(w/v)NaCl和pH6.0-6.5。泛醌-10是两个菌株中唯一的泛醌。H149T菌株的主要细胞脂肪酸为C16:0,求和特征3和求和特征8,而Z2T菌株的主要细胞脂肪酸为C17:1ω6c,求和特征3和求和特征8。菌株H149T和Z2T共享二磷脂酰甘油,磷脂酰甘油,磷脂酰乙醇胺,磷脂酰胆碱和鞘糖脂为主要极性脂质。16SrRNA基因序列同一性分析表明,H149T菌株与土木抗细菌KCTC23981T具有98.4%的最高序列同一性,菌株Z2T的含量为98.2%,QipengyuaniapacificaNZ-96T。基于16SrRNA基因和核心基因组序列的系统发育树显示,菌株H149T和Z2T在耳杆菌属和Qipengyuania属中形成了两个独立的进化枝,分别。菌株H149T的平均核苷酸同一性值为74.0-81.3%,与抗细菌型菌株的计算机DNA-DNA杂交值为18.5-23.1%,而Qipengyuania型菌株的Z2T值分别为73.3-78.7%和14.5-33.3%。H149T和Z2T菌株的基因组DNAGC含量分别为64.3和61.8%,分别。基于基因,基因组,系统发育,生理和化学分类学结果,菌株H149T(=KCTC8397T=MCCC1K08920T)和Z2T(=KCTC8396T=MCCC1K08946T)被认为代表了两种新的红杆菌科物种,其名称为Aurantiacibacter。11月。和浙江凤凤竹。11月。被提议,分别。
Two Gram-stain-negative, rod-shaped, non-motile, aerobic and carotenoid-producing strains, belonging to the family Erythrobacteraceae, designated as H149T and Z2T, were isolated from tidal flat sediment samples collected in Hainan and Zhejiang, PR
China, respectively. Growth of strain H149T occurred at 15-42 °C, 0-10.0 % (w/v) NaCl, and pH 6.0-8.5, with the optima at 35-37 °C, 3.0-3.5 % (w/v) NaCl and pH 7.0. Strain Z2T grew at 15-37 °C, 0-6.0 % (w/v) NaCl, and pH 6.0-9.5, with the optima at 25-30 °C, 0.5-1.0 % (w/v) NaCl and pH 6.0-6.5. Ubiquinone-10 was the sole ubiquinone in two strains. The predominant cellular fatty acids of strain H149T were C16 : 0, summed feature 3 and summed feature 8, while those of strain Z2T were C17 : 1 ω6c, summed feature 3 and summed feature 8. Strains H149T and Z2T shared diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid as major polar lipids. The 16S rRNA gene sequence identity analysis indicated that strain H149T had the highest sequence identity of 98.4 % with Aurantiacibacter odishensis KCTC 23981T, and strain Z2T had that of 98.2 % with Qipengyuania pacifica NZ-96T. Phylogenetic trees based on 16S rRNA gene and core-genome sequences revealed that strains H149T and Z2T formed two independent clades in the genera Aurantiacibacter and Qipengyuania, respectively. Strain H149T had average nucleotide identity values of 74.0-81.3 % and in silico DNA-DNA hybridization values of 18.5-23.1 % with Aurantiacibacter type strains, while strain Z2T had values of 73.3-78.7 % and 14.5-33.3 % with Qipengyuania type strains. The genomic DNA G+C contents of strains H149T and Z2T were 64.3 and 61.8 %, respectively. Based on the genetic, genomic, phylogenetic, physiological and chemotaxonomic results, strains H149T (=KCTC 8397T=MCCC 1K08920T) and Z2T (=KCTC 8396T=MCCC 1K08946T) are concluded to represent two novel Erythrobacteraceae species for which the names Aurantiacibacter hainanensis sp. nov. and Qipengyuania zhejiangensis sp. nov. are proposed, respectively.