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Abstract：
Culture-dependent and metagenomic binning techniques were employed to gain an insight into the diversification of gut bacteria in Rhinopithecius bieti, a highly endangered primate endemic to China. Our analyses revealed that Bacillota_A and Bacteroidota were the dominant phyla. These two phyla species are rich in carbohydrate active enzymes, which could provide nutrients and energy for their own or hosts\' survival under different circumstances. Among the culturable bacteria, one novel bacterium, designated as WQ 2009T, formed a distinct branch that had a low similarity to the known species in the family Sphingobacteriaceae, based on the phylogenetic analysis of its 16S rRNA gene sequence or phylogenomic analysis. The ANI, dDDH and AAI values between WQ 2009T and its most closely related strains S. kitahiroshimense 10CT, S. pakistanense NCCP-246T and S. faecium DSM 11690T were significantly lower than the accepted cut-off values for microbial species delineation. All results demonstrated that WQ 2009T represent a novel genus, for which names Rhinopithecimicrobium gen. nov. and Rhinopithecimicrobium faecis sp. nov. (Type strain WQ 2009T = CCTCC AA 2021153T = KCTC 82941T) are proposed.
摘要：
采用培养依赖性和宏基因组分级技术来深入了解Rhinopitheciusbieti中肠道细菌的多样化，中国特有的高度濒危的灵长类动物。我们的分析表明，芽孢杆菌A和拟杆菌是主要的门。这两个门物种富含碳水化合物活性酶，它们可以为自己或宿主在不同情况下的生存提供营养和能量。在可培养的细菌中，一种新的细菌，指定为WQ2009T，形成了一个独特的分支，与鞘杆菌科中的已知物种相似度很低，基于其16SrRNA基因序列的系统发育分析或系统发育分析。ANI,WQ2009T与其最密切相关的菌株Kitahiroshimense10CT之间的dDDH和AAI值，S.PakistanenseNCCP-246T和S.faeciumDSM11690T显着低于公认的微生物物种划分的临界值。所有结果表明，WQ2009T代表了一个新属，为此命名了莱茵霉素根。11月。和羊毛虫。11月。（提出了类型菌株WQ2009T=CCTCCAA2021153T=KCTC82941T）。
