PDF(Pubmed)
Abstract：
Three bacterial strains, FP250T, FP821, and FP53, were isolated from the rhizosphere soil of oilseed rape, licorice, and habanero pepper in Anhui Province, Xinjiang Uygur Autonomous Region, and Jiangsu Province, PR China, respectively. All strains were shown to grow at 4-37 °C and pH 6.0-9.0, and in the presence of 0-4.0 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences or housekeeping genes (16S rRNA, gyrB, rpoB, and rpoD) and phylogenomic analysis showed that strains FP250T, FP821, and FP53 belong to the genus Pseudomonas, and are closely related to Pseudomonas kilonensis DSM 13647T, Pseudomonas brassicacearum JCM 11938T, Pseudomonas viciae 11K1T, and Pseudomonas thivervalensis DSM 13194T. The DNA G+C content of strain FP205T was 59.8 mol%. The average nucleotide identity and digital DNA-DNA hybridization values of strain FP205T with the most closely related strain were 93.2 % and 51.4 %, respectively, which is well below the threshold for species differentiation. Strain FP205T contained summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) as major fatty acids, and diphosphatidylglycerol along with phosphatidylethanolamine and aminophospholipid as major polar lipids. The predominant isoprenoid quinone was ubiquinone-9. Based on these phenotypic, phylogenetic, and chemotaxonomic results, strain FP205T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas hefeiensis sp. nov. is proposed. The type strain is FP205T (=ACCC 62447T=JCM 35687T).
摘要：
三种细菌菌株，FP250T,从油菜的根际土壤中分离出FP821和FP53，甘草,和安徽的哈巴内罗胡椒，新疆维吾尔自治区,江苏省，中国公关,分别。所有菌株均显示在4-37°C和pH6.0-9.0以及0-4.0％（w/v）NaCl存在下生长。基于16SrRNA基因序列或管家基因的系统发育分析(16SrRNA，gyrB,rpoB,和rpoD)和系统基因组分析表明，菌株FP250T，FP821和FP53属于假单胞菌属，与Kilonensis假单胞菌DSM13647T密切相关，油菜初生假单胞菌JCM11938T,假单胞菌11K1T，和硫黄假单胞菌DSM13194T。菌株FP205T的DNAG+C含量为59.8mol%。FP205T菌株与最密切相关菌株的平均核苷酸同一性和数字DNA-DNA杂交值分别为93.2％和51.4％，分别,远低于物种分化的阈值。菌株FP205T包含求和特征3(C16:1ω6c和/或C16:1ω7c)，总特征8(C18:1ω7c和/或C18:1ω6c)作为主要脂肪酸，和二磷脂酰甘油以及磷脂酰乙醇胺和氨基磷脂作为主要的极性脂质。主要的类异戊二烯醌是泛醌-9。基于这些表型，系统发育，和化学分类学结果，菌株FP205T代表假单胞菌属的新物种，其名称为Hsefeiensis假单胞菌。11月。是提议的。菌株类型为FP205T(=ACCC62447T=JCM35687T)。
